1k0s
From Proteopedia
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'''Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima'''<br /> | '''Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Using protein from the hyperthermophile Thermotoga maritima, we have | + | Using protein from the hyperthermophile Thermotoga maritima, we have determined the solution structure of CheW, an essential component in the formation of the bacterial chemotaxis signaling complex. The overall fold is similar to the regulatory domain of the chemotaxis kinase CheA. In addition, interactions of CheW with CheA were monitored by nuclear magnetic resonance (NMR) techniques. The chemical shift perturbation data show the probable contacts that CheW makes with CheA. In combination with previous genetic data, the structure also suggests a possible binding site for the chemotaxis receptor. These results provide a structural basis for a model in which CheW acts as a molecular bridge between CheA and the cytoplasmic tails of the receptor. |
==About this Structure== | ==About this Structure== | ||
| - | 1K0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http:// | + | 1K0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
| - | [[Category: Dahlquist, F | + | [[Category: Dahlquist, F W.]] |
| - | [[Category: Griswold, I | + | [[Category: Griswold, I J.]] |
| - | [[Category: Simon, M | + | [[Category: Simon, M I.]] |
| - | [[Category: Swanson, R | + | [[Category: Swanson, R V.]] |
[[Category: Zhou, H.]] | [[Category: Zhou, H.]] | ||
[[Category: chew]] | [[Category: chew]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:51 2008'' |
Revision as of 11:28, 21 February 2008
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Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima
Overview
Using protein from the hyperthermophile Thermotoga maritima, we have determined the solution structure of CheW, an essential component in the formation of the bacterial chemotaxis signaling complex. The overall fold is similar to the regulatory domain of the chemotaxis kinase CheA. In addition, interactions of CheW with CheA were monitored by nuclear magnetic resonance (NMR) techniques. The chemical shift perturbation data show the probable contacts that CheW makes with CheA. In combination with previous genetic data, the structure also suggests a possible binding site for the chemotaxis receptor. These results provide a structural basis for a model in which CheW acts as a molecular bridge between CheA and the cytoplasmic tails of the receptor.
About this Structure
1K0S is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
The solution structure and interactions of CheW from Thermotoga maritima., Griswold IJ, Zhou H, Matison M, Swanson RV, McIntosh LP, Simon MI, Dahlquist FW, Nat Struct Biol. 2002 Feb;9(2):121-5. PMID:11799399
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