1k1g
From Proteopedia
(New page: 200px<br /> <applet load="1k1g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k1g" /> '''STRUCTURAL BASIS FOR RECOGNITION OF THE INT...) |
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| - | <applet load="1k1g" size=" | + | |
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'''STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1'''<br /> | '''STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During spliceosome assembly, splicing factor 1 (SF1) specifically | + | During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A. |
==About this Structure== | ==About this Structure== | ||
| - | 1K1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1K1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bottomley, M | + | [[Category: Bottomley, M J.]] |
[[Category: Houngninou-Molango, S.]] | [[Category: Houngninou-Molango, S.]] | ||
[[Category: Kramer, A.]] | [[Category: Kramer, A.]] | ||
[[Category: Liu, Z.]] | [[Category: Liu, Z.]] | ||
[[Category: Luyten, I.]] | [[Category: Luyten, I.]] | ||
| - | [[Category: Messias, A | + | [[Category: Messias, A C.]] |
[[Category: Sattler, M.]] | [[Category: Sattler, M.]] | ||
[[Category: Sprangers, R.]] | [[Category: Sprangers, R.]] | ||
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[[Category: star proteins]] | [[Category: star proteins]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:04 2008'' |
Revision as of 11:29, 21 February 2008
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STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1
Overview
During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A.
About this Structure
1K1G is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for recognition of the intron branch site RNA by splicing factor 1., Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, Zanier K, Kramer A, Sattler M, Science. 2001 Nov 2;294(5544):1098-102. PMID:11691992
Page seeded by OCA on Thu Feb 21 13:29:04 2008
