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1k1q
From Proteopedia
(New page: 200px<br /><applet load="1k1q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k1q, resolution 2.80Å" /> '''Crystal Structure of...) |
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| - | [[Image:1k1q.gif|left|200px]]<br /><applet load="1k1q" size=" | + | [[Image:1k1q.gif|left|200px]]<br /><applet load="1k1q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k1q, resolution 2.80Å" /> | caption="1k1q, resolution 2.80Å" /> | ||
'''Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus'''<br /> | '''Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A new group of error-prone DNA polymerases overcomes the blockage posed to | + | A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations. |
==About this Structure== | ==About this Structure== | ||
| - | 1K1Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http:// | + | 1K1Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
| - | [[Category: Goodman, M | + | [[Category: Goodman, M F.]] |
[[Category: Pham, P.]] | [[Category: Pham, P.]] | ||
| - | [[Category: Silvian, L | + | [[Category: Silvian, L F.]] |
| - | [[Category: Toth, E | + | [[Category: Toth, E A.]] |
[[Category: dna polymerase]] | [[Category: dna polymerase]] | ||
[[Category: error-prone polymerase]] | [[Category: error-prone polymerase]] | ||
[[Category: lesion-bypass polymerase]] | [[Category: lesion-bypass polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:09 2008'' |
Revision as of 11:29, 21 February 2008
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Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus
Overview
A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.
About this Structure
1K1Q is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus., Silvian LF, Toth EA, Pham P, Goodman MF, Ellenberger T, Nat Struct Biol. 2001 Nov;8(11):984-9. PMID:11685247
Page seeded by OCA on Thu Feb 21 13:29:09 2008
