1k2d
From Proteopedia
(New page: 200px<br /><applet load="1k2d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2d, resolution 2.2Å" /> '''Crystal structure of ...) |
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| - | [[Image:1k2d.jpg|left|200px]]<br /><applet load="1k2d" size=" | + | [[Image:1k2d.jpg|left|200px]]<br /><applet load="1k2d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k2d, resolution 2.2Å" /> | caption="1k2d, resolution 2.2Å" /> | ||
'''Crystal structure of the autoimmune MHC class II I-Au complexed with myelin basic protein 1-11 at 2.2A'''<br /> | '''Crystal structure of the autoimmune MHC class II I-Au complexed with myelin basic protein 1-11 at 2.2A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Murine experimental allergic encephalomyelitis (EAE) is a useful model for | + | Murine experimental allergic encephalomyelitis (EAE) is a useful model for the demyelinating, autoimmune disease multiple sclerosis. In the EAE system, the immunodominant N-terminal epitope of myelin basic protein (MBP) is an unusually short, weakly binding peptide antigen which elicits highly biased TCR chain usage. In the 2.2 A crystal structure of I-A(u)/MBP1-11 complex, only MBP residues 1-7 are bound toward one end of the peptide binding cleft. The fourth residue of MBP1-11 is located in an incompatible p6 pocket of I-A(u), thus explaining the short half-life of I-A(u) complexed with Ac1-11. MBP peptides extended at the C terminus of Ac1-11 result in dramatic affinity increases, likely attributed to register shifting to a higher affinity cryptic epitope, which could potentially mask the presentation of the immunodominant MBP1-11 peptide during thymic education. |
==About this Structure== | ==About this Structure== | ||
| - | 1K2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NDG and NAG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1K2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NDG:'>NDG</scene> and <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Garcia, K | + | [[Category: Garcia, K C.]] |
| - | [[Category: He, X | + | [[Category: He, X L.]] |
[[Category: Radu, C.]] | [[Category: Radu, C.]] | ||
| - | [[Category: Ward, E | + | [[Category: Ward, E S.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: NDG]] | [[Category: NDG]] | ||
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[[Category: unique register]] | [[Category: unique register]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:19 2008'' |
Revision as of 11:29, 21 February 2008
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Crystal structure of the autoimmune MHC class II I-Au complexed with myelin basic protein 1-11 at 2.2A
Overview
Murine experimental allergic encephalomyelitis (EAE) is a useful model for the demyelinating, autoimmune disease multiple sclerosis. In the EAE system, the immunodominant N-terminal epitope of myelin basic protein (MBP) is an unusually short, weakly binding peptide antigen which elicits highly biased TCR chain usage. In the 2.2 A crystal structure of I-A(u)/MBP1-11 complex, only MBP residues 1-7 are bound toward one end of the peptide binding cleft. The fourth residue of MBP1-11 is located in an incompatible p6 pocket of I-A(u), thus explaining the short half-life of I-A(u) complexed with Ac1-11. MBP peptides extended at the C terminus of Ac1-11 result in dramatic affinity increases, likely attributed to register shifting to a higher affinity cryptic epitope, which could potentially mask the presentation of the immunodominant MBP1-11 peptide during thymic education.
About this Structure
1K2D is a Protein complex structure of sequences from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural snapshot of aberrant antigen presentation linked to autoimmunity: the immunodominant epitope of MBP complexed with I-Au., He XL, Radu C, Sidney J, Sette A, Ward ES, Garcia KC, Immunity. 2002 Jul;17(1):83-94. PMID:12150894
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