1k2l
From Proteopedia
(New page: 200px<br /><applet load="1k2l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2l, resolution 2.40Å" /> '''STRUCTURAL CHARACTER...) |
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| - | [[Image:1k2l.gif|left|200px]]<br /><applet load="1k2l" size=" | + | [[Image:1k2l.gif|left|200px]]<br /><applet load="1k2l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k2l, resolution 2.40Å" /> | caption="1k2l, resolution 2.40Å" /> | ||
'''STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX'''<br /> | '''STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the single-crystal X-ray structure for the complex of the | + | We report the single-crystal X-ray structure for the complex of the bisacridine bis-(9-aminooctyl(2-(dimethylaminoethyl)acridine-4-carboxamide)) with the oligonucleotide d(CGTACG)(2) to a resolution of 2.4A. Solution studies with closed circular DNA show this compound to be a bisintercalating threading agent, but so far we have no crystallographic or NMR structural data conforming to the model of contiguous intercalation within the same duplex. Here, with the hexameric duplex d(CGTACG), the DNA is observed to undergo a terminal cytosine base exchange to yield an unusual guanine quadruplex intercalation site through which the bisacridine threads its octamethylene linker to fuse two DNA duplexes. The 4-carboxamide side-chains form anchoring hydrogen-bonding interactions with guanine O6 atoms on each side of the quadruplex. This higher-order DNA structure provides insight into an unexpected property of bisintercalating threading agents, and suggests the idea of targeting such compounds specifically at four-way DNA junctions. |
==About this Structure== | ==About this Structure== | ||
| - | 1K2L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BDC and CO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1K2L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BDC:'>BDC</scene> and <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Adams, A.]] | [[Category: Adams, A.]] | ||
| - | [[Category: Cardin, C | + | [[Category: Cardin, C J.]] |
| - | [[Category: Denny, W | + | [[Category: Denny, W A.]] |
| - | [[Category: Powell, H | + | [[Category: Powell, H R.]] |
| - | [[Category: Teixeira, S | + | [[Category: Teixeira, S C.M.]] |
| - | [[Category: Thorpe, J | + | [[Category: Thorpe, J H.]] |
| - | [[Category: Todd, A | + | [[Category: Todd, A K.]] |
| - | [[Category: Wakelin, L | + | [[Category: Wakelin, L P.G.]] |
[[Category: BDC]] | [[Category: BDC]] | ||
[[Category: CO]] | [[Category: CO]] | ||
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[[Category: quadruplex]] | [[Category: quadruplex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:24 2008'' |
Revision as of 11:29, 21 February 2008
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STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX
Overview
We report the single-crystal X-ray structure for the complex of the bisacridine bis-(9-aminooctyl(2-(dimethylaminoethyl)acridine-4-carboxamide)) with the oligonucleotide d(CGTACG)(2) to a resolution of 2.4A. Solution studies with closed circular DNA show this compound to be a bisintercalating threading agent, but so far we have no crystallographic or NMR structural data conforming to the model of contiguous intercalation within the same duplex. Here, with the hexameric duplex d(CGTACG), the DNA is observed to undergo a terminal cytosine base exchange to yield an unusual guanine quadruplex intercalation site through which the bisacridine threads its octamethylene linker to fuse two DNA duplexes. The 4-carboxamide side-chains form anchoring hydrogen-bonding interactions with guanine O6 atoms on each side of the quadruplex. This higher-order DNA structure provides insight into an unexpected property of bisintercalating threading agents, and suggests the idea of targeting such compounds specifically at four-way DNA junctions.
About this Structure
1K2L is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural characterisation of bisintercalation in higher-order DNA at a junction-like quadruplex., Teixeira SC, Thorpe JH, Todd AK, Powell HR, Adams A, Wakelin LP, Denny WA, Cardin CJ, J Mol Biol. 2002 Oct 18;323(2):167-71. PMID:12381312
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