1k3s
From Proteopedia
(New page: 200px<br /><applet load="1k3s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k3s, resolution 1.9Å" /> '''Type III Secretion Ch...) |
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| - | [[Image:1k3s.jpg|left|200px]]<br /><applet load="1k3s" size=" | + | [[Image:1k3s.jpg|left|200px]]<br /><applet load="1k3s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k3s, resolution 1.9Å" /> | caption="1k3s, resolution 1.9Å" /> | ||
'''Type III Secretion Chaperone SigE'''<br /> | '''Type III Secretion Chaperone SigE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Several Gram-negative bacterial pathogens have evolved a type III | + | Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones. |
==About this Structure== | ==About this Structure== | ||
| - | 1K3S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1K3S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Salmonella enterica]] | [[Category: Salmonella enterica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bertero, M | + | [[Category: Bertero, M G.]] |
[[Category: Creagh, L.]] | [[Category: Creagh, L.]] | ||
| - | [[Category: Finlay, B | + | [[Category: Finlay, B B.]] |
| - | [[Category: Frey, E | + | [[Category: Frey, E A.]] |
[[Category: Lim, D.]] | [[Category: Lim, D.]] | ||
[[Category: Luo, Y.]] | [[Category: Luo, Y.]] | ||
| - | [[Category: Marcus, S | + | [[Category: Marcus, S L.]] |
| - | [[Category: Pfuetzner, R | + | [[Category: Pfuetzner, R A.]] |
| - | [[Category: Strynadka, N | + | [[Category: Strynadka, N C.J.]] |
| - | [[Category: Wenk, M | + | [[Category: Wenk, M R.]] |
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: chaperone]] | [[Category: chaperone]] | ||
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[[Category: type iii]] | [[Category: type iii]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:51 2008'' |
Revision as of 11:29, 21 February 2008
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Type III Secretion Chaperone SigE
Overview
Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones.
About this Structure
1K3S is a Single protein structure of sequence from Salmonella enterica with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and biochemical characterization of the type III secretion chaperones CesT and SigE., Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC, Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:11685226
Page seeded by OCA on Thu Feb 21 13:29:51 2008
Categories: Salmonella enterica | Single protein | Bertero, M G. | Creagh, L. | Finlay, B B. | Frey, E A. | Lim, D. | Luo, Y. | Marcus, S L. | Pfuetzner, R A. | Strynadka, N C.J. | Wenk, M R. | PO4 | Chaperone | Secretion | Sige | Type iii
