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1k4j
From Proteopedia
(New page: 200px<br /><applet load="1k4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k4j, resolution 2.5Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1k4j.jpg|left|200px]]<br /><applet load="1k4j" size=" | + | [[Image:1k4j.jpg|left|200px]]<br /><applet load="1k4j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k4j, resolution 2.5Å" /> | caption="1k4j, resolution 2.5Å" /> | ||
'''Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate'''<br /> | '''Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Synthesis and detection of acyl-homoserine lactones (AHLs) enables many | + | Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed. |
==About this Structure== | ==About this Structure== | ||
| - | 1K4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii] with REO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1K4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii] with <scene name='pdbligand=REO:'>REO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pantoea stewartii subsp. stewartii]] | [[Category: Pantoea stewartii subsp. stewartii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bodman, S | + | [[Category: Bodman, S Beck von.]] |
| - | [[Category: Churchill, M | + | [[Category: Churchill, M E.A.]] |
| - | [[Category: Minogue, T | + | [[Category: Minogue, T D.]] |
| - | [[Category: Val, D | + | [[Category: Val, D L.]] |
| - | [[Category: Watson, W | + | [[Category: Watson, W T.]] |
[[Category: REO]] | [[Category: REO]] | ||
[[Category: acyl-homoserinelactone synthase]] | [[Category: acyl-homoserinelactone synthase]] | ||
| Line 26: | Line 26: | ||
[[Category: quorum sensing]] | [[Category: quorum sensing]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:05 2008'' |
Revision as of 11:30, 21 February 2008
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Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate
Overview
Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.
About this Structure
1K4J is a Single protein structure of sequence from Pantoea stewartii subsp. stewartii with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing., Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME, Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774
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