1k5m
From Proteopedia
(New page: 200px<br /> <applet load="1k5m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k5m, resolution 2.7Å" /> '''Crystal Structure of...) |
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| - | [[Image:1k5m.gif|left|200px]]<br /> | + | [[Image:1k5m.gif|left|200px]]<br /><applet load="1k5m" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1k5m" size=" | + | |
caption="1k5m, resolution 2.7Å" /> | caption="1k5m, resolution 2.7Å" /> | ||
'''Crystal Structure of a Human Rhinovirus Type 14:Human Immunodeficiency Virus Type 1 V3 Loop Chimeric Virus MN-III-2'''<br /> | '''Crystal Structure of a Human Rhinovirus Type 14:Human Immunodeficiency Virus Type 1 V3 Loop Chimeric Virus MN-III-2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the 2.7 A resolution structure of a chimeric rhinovirus, MN-III-2, that displays part of the HIV-1 gp120 V3 loop and elicits | + | We report the 2.7 A resolution structure of a chimeric rhinovirus, MN-III-2, that displays part of the HIV-1 gp120 V3 loop and elicits HIV-neutralizing antibodies. The V3 loop insert is dominated by two type I beta turns. The structures of two adjacent tripeptides resemble those of analogous segments in three Fab/V3 loop peptide complexes. Although two of the three corresponding antibodies bind and neutralize MN-III-2 well, only one of the three can bind without significant rearrangement. These results suggest that the V3 loop insert: (1) can share some local conformational similarity to V3 loop sequences presented on different structural frameworks; (2) must be able to adopt multiple conformations, even in a relatively constrained environment; and (3) may mimic the conformational variability of the epitope on HIV-1, increasing the likelihood of eliciting appropriate neutralizing immune responses. |
==About this Structure== | ==About this Structure== | ||
| - | 1K5M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with SPH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1K5M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with <scene name='pdbligand=SPH:'>SPH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Arnold, E.]] | [[Category: Arnold, E.]] | ||
| - | [[Category: Arnold, G | + | [[Category: Arnold, G F.]] |
[[Category: Ding, J.]] | [[Category: Ding, J.]] | ||
| - | [[Category: Geisler, S | + | [[Category: Geisler, S C.]] |
[[Category: Ma, X.]] | [[Category: Ma, X.]] | ||
| - | [[Category: Smith, A | + | [[Category: Smith, A D.]] |
[[Category: SPH]] | [[Category: SPH]] | ||
[[Category: beta turns]] | [[Category: beta turns]] | ||
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[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:28 2008'' |
Revision as of 11:30, 21 February 2008
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Crystal Structure of a Human Rhinovirus Type 14:Human Immunodeficiency Virus Type 1 V3 Loop Chimeric Virus MN-III-2
Overview
We report the 2.7 A resolution structure of a chimeric rhinovirus, MN-III-2, that displays part of the HIV-1 gp120 V3 loop and elicits HIV-neutralizing antibodies. The V3 loop insert is dominated by two type I beta turns. The structures of two adjacent tripeptides resemble those of analogous segments in three Fab/V3 loop peptide complexes. Although two of the three corresponding antibodies bind and neutralize MN-III-2 well, only one of the three can bind without significant rearrangement. These results suggest that the V3 loop insert: (1) can share some local conformational similarity to V3 loop sequences presented on different structural frameworks; (2) must be able to adopt multiple conformations, even in a relatively constrained environment; and (3) may mimic the conformational variability of the epitope on HIV-1, increasing the likelihood of eliciting appropriate neutralizing immune responses.
About this Structure
1K5M is a Protein complex structure of sequences from Human rhinovirus 10 with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a human rhinovirus that displays part of the HIV-1 V3 loop and induces neutralizing antibodies against HIV-1., Ding J, Smith AD, Geisler SC, Ma X, Arnold GF, Arnold E, Structure. 2002 Jul;10(7):999-1011. PMID:12121655
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