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1k6d
From Proteopedia
(New page: 200px<br /><applet load="1k6d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k6d, resolution 1.90Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size=" | + | [[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k6d, resolution 1.90Å" /> | caption="1k6d, resolution 1.90Å" /> | ||
'''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''<br /> | '''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The automation of protein structure determination is an essential | + | The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal. |
==About this Structure== | ==About this Structure== | ||
| - | 1K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http:// | + | 1K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Korolev, S.]] | [[Category: Korolev, S.]] | ||
[[Category: Koroleva, O.]] | [[Category: Koroleva, O.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Petterson, K.]] | [[Category: Petterson, K.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:36 2008'' |
Revision as of 11:30, 21 February 2008
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CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT
Overview
The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
About this Structure
1K6D is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Acetate CoA-transferase, with EC number 2.8.3.8 Full crystallographic information is available from OCA.
Reference
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473
Page seeded by OCA on Thu Feb 21 13:30:36 2008
Categories: Acetate CoA-transferase | Escherichia coli | Single protein | Collart, F. | Dementieva, I. | Joachimiak, A. | Korolev, S. | Koroleva, O. | MCSG, Midwest Center for Structural Genomics. | Petterson, K. | MG | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Structural genomics
