1k6g
From Proteopedia
(New page: 200px<br /><applet load="1k6g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k6g" /> '''Solution Structure of Conserved AGNN Tetralo...) |
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'''Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing'''<br /> | '''Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and | + | Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage. |
==About this Structure== | ==About this Structure== | ||
| - | 1K6G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1K6G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6G OCA]. |
==Reference== | ==Reference== | ||
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11743001 11743001] | Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11743001 11743001] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Elela, S | + | [[Category: Elela, S Abou.]] |
[[Category: Fourmy, D.]] | [[Category: Fourmy, D.]] | ||
[[Category: Lamontagne, B.]] | [[Category: Lamontagne, B.]] | ||
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[[Category: rna tetraloop]] | [[Category: rna tetraloop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:41 2008'' |
Revision as of 11:30, 21 February 2008
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Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing
Overview
Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.
About this Structure
1K6G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:11743001
Page seeded by OCA on Thu Feb 21 13:30:41 2008
