1k6x
From Proteopedia
(New page: 200px<br /><applet load="1k6x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k6x, resolution 1.50Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1k6x.gif|left|200px]]<br /><applet load="1k6x" size=" | + | [[Image:1k6x.gif|left|200px]]<br /><applet load="1k6x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k6x, resolution 1.50Å" /> | caption="1k6x, resolution 1.50Å" /> | ||
'''Crystal structure of Nmra, a negative transcriptional regulator in complex with NAD at 1.5 A resolution (Trigonal form)'''<br /> | '''Crystal structure of Nmra, a negative transcriptional regulator in complex with NAD at 1.5 A resolution (Trigonal form)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NmrA is a negative transcriptional regulator involved in the | + | NmrA is a negative transcriptional regulator involved in the post-translational modulation of the GATA-type transcription factor AreA, forming part of a system controlling nitrogen metabolite repression in various fungi. X-ray structures of two NmrA crystal forms, both to 1.8 A resolution, show NmrA consists of two domains, including a Rossmann fold. NmrA shows an unexpected similarity to the short-chain dehydrogenase/reductase (SDR) family, with the closest relationship to UDP-galactose 4-epimerase. We show that NAD binds to NmrA, a previously unreported nucleotide binding property for this protein. NmrA is unlikely to be an active dehydrogenase, however, as the conserved catalytic tyrosine in SDRs is absent in NmrA, and thus the nucleotide binding to NmrA could have a regulatory function. Our results suggest that other transcription factors possess the SDR fold with functions including RNA binding. The SDR fold appears to have been adapted for other roles including non-enzymatic control functions such as transcriptional regulation and is likely to be more widespread than previously recognized. |
==About this Structure== | ==About this Structure== | ||
| - | 1K6X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with CL and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1K6X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6X OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Cocklin, S.]] | [[Category: Cocklin, S.]] | ||
[[Category: Dodds, A.]] | [[Category: Dodds, A.]] | ||
| - | [[Category: Hawkins, A | + | [[Category: Hawkins, A R.]] |
| - | [[Category: Lamb, H | + | [[Category: Lamb, H K.]] |
[[Category: Leslie, K.]] | [[Category: Leslie, K.]] | ||
| - | [[Category: Nichols, C | + | [[Category: Nichols, C E.]] |
[[Category: Ren, J.]] | [[Category: Ren, J.]] | ||
| - | [[Category: Stammers, D | + | [[Category: Stammers, D K.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
[[Category: rossmann fold transcriptional regulation short chain dehydrogenase reductase nadh binding]] | [[Category: rossmann fold transcriptional regulation short chain dehydrogenase reductase nadh binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:47 2008'' |
Revision as of 11:30, 21 February 2008
|
Crystal structure of Nmra, a negative transcriptional regulator in complex with NAD at 1.5 A resolution (Trigonal form)
Overview
NmrA is a negative transcriptional regulator involved in the post-translational modulation of the GATA-type transcription factor AreA, forming part of a system controlling nitrogen metabolite repression in various fungi. X-ray structures of two NmrA crystal forms, both to 1.8 A resolution, show NmrA consists of two domains, including a Rossmann fold. NmrA shows an unexpected similarity to the short-chain dehydrogenase/reductase (SDR) family, with the closest relationship to UDP-galactose 4-epimerase. We show that NAD binds to NmrA, a previously unreported nucleotide binding property for this protein. NmrA is unlikely to be an active dehydrogenase, however, as the conserved catalytic tyrosine in SDRs is absent in NmrA, and thus the nucleotide binding to NmrA could have a regulatory function. Our results suggest that other transcription factors possess the SDR fold with functions including RNA binding. The SDR fold appears to have been adapted for other roles including non-enzymatic control functions such as transcriptional regulation and is likely to be more widespread than previously recognized.
About this Structure
1K6X is a Single protein structure of sequence from Emericella nidulans with and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases., Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR, EMBO J. 2001 Dec 3;20(23):6619-26. PMID:11726498
Page seeded by OCA on Thu Feb 21 13:30:47 2008
