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1hl7
From Proteopedia
(New page: 200px<br /> <applet load="1hl7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hl7, resolution 1.73Å" /> '''GAMMA LACTAMASE FRO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HL7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Microbacterium Microbacterium]] with BD1 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HL7 OCA]]. | + | 1HL7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Microbacterium Microbacterium]] with BD1 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HL7 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: tetrahedral intermediate]] | [[Category: tetrahedral intermediate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:29:48 2007'' |
Revision as of 12:25, 30 October 2007
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GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE
Overview
The structure of the recombinant (-) gamma-lactamase from an, Aureobacterium species has been solved at 1.73A resolution in the cubic, space group F23 with unit cell parameters a=b=c=240.6A. The trimeric, enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor, free haloperoxidases. The structure has been solved in complex with a, covalently bound ligand originating from the host cell and also in the, unligated form. The associated density in the former structure has been, interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo, [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the, catalytic Ser98. Soaks of these crystals with the industrial substrate, gamma-lactam or its structural analogue, norcamphor, result in the, displacement of the ... [(full description)]
About this Structure
1HL7 is a [Single protein] structure of sequence from [Microbacterium] with BD1 as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810
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