1k9y

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(New page: 200px<br /><applet load="1k9y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k9y, resolution 1.90&Aring;" /> '''The PAPase Hal2p com...)
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'''The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate'''<br />
'''The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate'''<br />
==Overview==
==Overview==
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Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable, attention since they have been proposed as targets for lithium therapy in, the treatment of manic-depressive patients. The members of this enzyme, superfamily display low levels of sequence identity while possessing a, common fold and active site. Extensive structural and biochemical data, demonstrate the direct involvement of two metal ions in catalysis, and, show that lithium exerts its inhibitory action by blocking the products at, the active site. By exploiting the different inhibitory properties of, magnesium and calcium, we have been able to solve the X-ray structures of, the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in, complex with its substrate and with its products. The structural, comparison of these complexes provides a 3D picture of the different, stages of the catalytic cycle. This gives new insights into the, understanding of the biological function of this group of enzymes and, their lithium inhibition, and should assist in the design of improved, inhibitors of therapeutic value.
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Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable attention since they have been proposed as targets for lithium therapy in the treatment of manic-depressive patients. The members of this enzyme superfamily display low levels of sequence identity while possessing a common fold and active site. Extensive structural and biochemical data demonstrate the direct involvement of two metal ions in catalysis, and show that lithium exerts its inhibitory action by blocking the products at the active site. By exploiting the different inhibitory properties of magnesium and calcium, we have been able to solve the X-ray structures of the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in complex with its substrate and with its products. The structural comparison of these complexes provides a 3D picture of the different stages of the catalytic cycle. This gives new insights into the understanding of the biological function of this group of enzymes and their lithium inhibition, and should assist in the design of improved inhibitors of therapeutic value.
==About this Structure==
==About this Structure==
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1K9Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG, PO4, AMP and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3'(2'),5'-bisphosphate_nucleotidase 3'(2'),5'-bisphosphate nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.7 3.1.3.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K9Y OCA].
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1K9Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=AMP:'>AMP</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3'(2'),5'-bisphosphate_nucleotidase 3'(2'),5'-bisphosphate nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.7 3.1.3.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9Y OCA].
==Reference==
==Reference==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Albert, A.]]
[[Category: Albert, A.]]
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[[Category: Blundell, T.L.]]
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[[Category: Blundell, T L.]]
[[Category: Patel, S.]]
[[Category: Patel, S.]]
[[Category: AMP]]
[[Category: AMP]]
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[[Category: salt tolerance]]
[[Category: salt tolerance]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:59:17 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:44 2008''

Revision as of 11:31, 21 February 2008


1k9y, resolution 1.90Å

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The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate

Overview

Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable attention since they have been proposed as targets for lithium therapy in the treatment of manic-depressive patients. The members of this enzyme superfamily display low levels of sequence identity while possessing a common fold and active site. Extensive structural and biochemical data demonstrate the direct involvement of two metal ions in catalysis, and show that lithium exerts its inhibitory action by blocking the products at the active site. By exploiting the different inhibitory properties of magnesium and calcium, we have been able to solve the X-ray structures of the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in complex with its substrate and with its products. The structural comparison of these complexes provides a 3D picture of the different stages of the catalytic cycle. This gives new insights into the understanding of the biological function of this group of enzymes and their lithium inhibition, and should assist in the design of improved inhibitors of therapeutic value.

About this Structure

1K9Y is a Single protein structure of sequence from Saccharomyces cerevisiae with , , and as ligands. Active as 3'(2'),5'-bisphosphate nucleotidase, with EC number 3.1.3.7 Full crystallographic information is available from OCA.

Reference

Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases., Patel S, Martinez-Ripoll M, Blundell TL, Albert A, J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:12126627

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