This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1kaw
From Proteopedia
(New page: 200px<br /><applet load="1kaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kaw, resolution 2.9Å" /> '''STRUCTURE OF SINGLE S...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1kaw.jpg|left|200px]]<br /><applet load="1kaw" size=" | + | [[Image:1kaw.jpg|left|200px]]<br /><applet load="1kaw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kaw, resolution 2.9Å" /> | caption="1kaw, resolution 2.9Å" /> | ||
'''STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)'''<br /> | '''STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the tetrameric DNA-binding domain of the | + | The crystal structure of the tetrameric DNA-binding domain of the single-stranded DNA binding protein from Escherichia coli was determined at a resolution of 2.9 A using multiwavelength anomalous dispersion. Each monomer in the tetramer is topologically similar to an oligomer-binding fold. Two monomers each contribute three beta-strands to a single six-stranded beta-sheet to form a dimer. Two dimer-dimer interfaces are observed within the crystal. One of these stabilizes the tetramer in solution. The other interface promotes a superhelical structure within the crystal that may reflect tetramer-tetramer interactions involved in the positive cooperative binding of the single-stranded DNA-binding protein to single-stranded DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1KAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1KAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAW OCA]. |
==Reference== | ==Reference== | ||
| Line 19: | Line 19: | ||
[[Category: ssb]] | [[Category: ssb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:00 2008'' |
Revision as of 11:32, 21 February 2008
|
STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)
Overview
The crystal structure of the tetrameric DNA-binding domain of the single-stranded DNA binding protein from Escherichia coli was determined at a resolution of 2.9 A using multiwavelength anomalous dispersion. Each monomer in the tetramer is topologically similar to an oligomer-binding fold. Two monomers each contribute three beta-strands to a single six-stranded beta-sheet to form a dimer. Two dimer-dimer interfaces are observed within the crystal. One of these stabilizes the tetramer in solution. The other interface promotes a superhelical structure within the crystal that may reflect tetramer-tetramer interactions involved in the positive cooperative binding of the single-stranded DNA-binding protein to single-stranded DNA.
About this Structure
1KAW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution., Raghunathan S, Ricard CS, Lohman TM, Waksman G, Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6652-7. PMID:9192620
Page seeded by OCA on Thu Feb 21 13:32:00 2008
