2ca6
From Proteopedia
(New page: 200px<br /> <applet load="2ca6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ca6, resolution 2.20Å" /> '''MIRAS STRUCTURE DET...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CA6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CA6 OCA]]. | + | 2CA6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CA6 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: signaling regulator]] | [[Category: signaling regulator]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:31:22 2007'' |
Revision as of 12:26, 30 October 2007
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MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS
Overview
The structure of Rna1p was originally solved to 2.7 A resolution by MIRAS, from crystals with partial hemihedral twinning in space group I4(1), [Hillig et al. (1999), Mol. Cell, 3, 781-791] by finding a low-twinned, native crystal (twin fraction alpha=0.06) and after twin correction of all, data sets. Rna1p crystals have now been used to examine how far twinning, and twin correction affect MIR phasing with a higher resolution but highly, twinned native data set. Even high hemihedral twinning [alphanative=0.39, alphaderivative=0.24] would not have hindered heavy-atom site, identification of strong derivatives using difference Patterson maps., However, a weaker derivative could have been missed and refinement would, have stalled at high R values had twinning not been identified and, ... [(full description)]
About this Structure
2CA6 is a [Single protein] structure of sequence from [Schizosaccharomyces pombe] with SO4 as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Detecting and overcoming hemihedral twinning during the MIR structure determination of Rna1p., Hillig RC, Renault L, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):750-65. Epub 2006, Jun 20. PMID:16790931
Page seeded by OCA on Tue Oct 30 14:31:22 2007
Categories: Schizosaccharomyces pombe | Single protein | Hillig, R.C. | Renault, L. | SO4 | Gap | Gtpase activation | Gtpase-activating protein | Hemihedral twinning | Leucine-rich repeat protein | Lrr | Merohedral twinning | Merohedry | Nuclear transport | Rangap | Rna1p | Signaling activator | Signaling protein | Signaling regulator
