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1ki0

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(New page: 200px<br /> <applet load="1ki0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ki0, resolution 1.75&Aring;" /> '''The X-ray Structure...)
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<applet load="1ki0" size="450" color="white" frame="true" align="right" spinBox="true"
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'''The X-ray Structure of Human Angiostatin'''<br />
'''The X-ray Structure of Human Angiostatin'''<br />
==Overview==
==Overview==
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Angiogenesis inhibitors have gained much public attention recently as, anti-cancer agents and several are currently in clinical trials, including, angiostatin (Phase I, Thomas Jefferson University Hospital, Philadelphia, PA). We report here the bowl-shaped structure of angiostatin kringles 1-3, the first multi-kringle structure to be determined. All three kringle, lysine-binding sites contain a bound bicine molecule of crystallization, while the former of kringle 2 and kringle 3 are cofacial. Moreover, the, separation of the kringle 2 and kringle 3 lysiner binding sites is, sufficient to accommodate the alpha-helix of the 30 residue peptide VEK-30, found in the kringle 2/VEK-30 complex. Together the three kringles produce, a central cavity suggestive of a unique domain where they may function in, concert.
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Angiogenesis inhibitors have gained much public attention recently as anti-cancer agents and several are currently in clinical trials, including angiostatin (Phase I, Thomas Jefferson University Hospital, Philadelphia, PA). We report here the bowl-shaped structure of angiostatin kringles 1-3, the first multi-kringle structure to be determined. All three kringle lysine-binding sites contain a bound bicine molecule of crystallization while the former of kringle 2 and kringle 3 are cofacial. Moreover, the separation of the kringle 2 and kringle 3 lysiner binding sites is sufficient to accommodate the alpha-helix of the 30 residue peptide VEK-30 found in the kringle 2/VEK-30 complex. Together the three kringles produce a central cavity suggestive of a unique domain where they may function in concert.
==Disease==
==Disease==
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==About this Structure==
==About this Structure==
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1KI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BCN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KI0 OCA].
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1KI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BCN:'>BCN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KI0 OCA].
==Reference==
==Reference==
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[[Category: Plasmin]]
[[Category: Plasmin]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Abad, M.C.]]
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[[Category: Abad, M C.]]
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[[Category: Arni, R.K.]]
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[[Category: Arni, R K.]]
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[[Category: Castellino, F.J.]]
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[[Category: Castellino, F J.]]
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[[Category: Geiger, J.H.]]
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[[Category: Geiger, J H.]]
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[[Category: Grella, D.K.]]
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[[Category: Grella, D K.]]
[[Category: Tulinsky, A.]]
[[Category: Tulinsky, A.]]
[[Category: BCN]]
[[Category: BCN]]
[[Category: kringle domains]]
[[Category: kringle domains]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:51:10 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:22 2008''

Revision as of 11:34, 21 February 2008


1ki0, resolution 1.75Å

Drag the structure with the mouse to rotate

The X-ray Structure of Human Angiostatin

Contents

Overview

Angiogenesis inhibitors have gained much public attention recently as anti-cancer agents and several are currently in clinical trials, including angiostatin (Phase I, Thomas Jefferson University Hospital, Philadelphia, PA). We report here the bowl-shaped structure of angiostatin kringles 1-3, the first multi-kringle structure to be determined. All three kringle lysine-binding sites contain a bound bicine molecule of crystallization while the former of kringle 2 and kringle 3 are cofacial. Moreover, the separation of the kringle 2 and kringle 3 lysiner binding sites is sufficient to accommodate the alpha-helix of the 30 residue peptide VEK-30 found in the kringle 2/VEK-30 complex. Together the three kringles produce a central cavity suggestive of a unique domain where they may function in concert.

Disease

Known diseases associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]

About this Structure

1KI0 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Plasmin, with EC number 3.4.21.7 Full crystallographic information is available from OCA.

Reference

The X-ray crystallographic structure of the angiogenesis inhibitor angiostatin., Abad MC, Arni RK, Grella DK, Castellino FJ, Tulinsky A, Geiger JH, J Mol Biol. 2002 May 10;318(4):1009-17. PMID:12054798

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