1kil
From Proteopedia
(New page: 200px<br /><applet load="1kil" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kil, resolution 2.30Å" /> '''Three-dimensional st...) |
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| - | [[Image:1kil.gif|left|200px]]<br /><applet load="1kil" size=" | + | [[Image:1kil.gif|left|200px]]<br /><applet load="1kil" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kil, resolution 2.30Å" /> | caption="1kil, resolution 2.30Å" /> | ||
'''Three-dimensional structure of the complexin/SNARE complex'''<br /> | '''Three-dimensional structure of the complexin/SNARE complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During neurotransmitter release, the neuronal SNARE proteins | + | During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release. |
==About this Structure== | ==About this Structure== | ||
| - | 1KIL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KIL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Machius, M.]] | [[Category: Machius, M.]] | ||
[[Category: Rizo, J.]] | [[Category: Rizo, J.]] | ||
| - | [[Category: Sudhof, T | + | [[Category: Sudhof, T C.]] |
[[Category: Tomchick, D.]] | [[Category: Tomchick, D.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: helix bound to four helix bundle]] | [[Category: helix bound to four helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:32 2008'' |
Revision as of 11:34, 21 February 2008
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Three-dimensional structure of the complexin/SNARE complex
Overview
During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release.
About this Structure
1KIL is a Protein complex structure of sequences from Homo sapiens and Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the complexin/SNARE complex., Chen X, Tomchick DR, Kovrigin E, Arac D, Machius M, Sudhof TC, Rizo J, Neuron. 2002 Jan 31;33(3):397-409. PMID:11832227
Page seeded by OCA on Thu Feb 21 13:34:32 2008
