1kj2
From Proteopedia
(New page: 200px<br /><applet load="1kj2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kj2, resolution 2.71Å" /> '''Murine Alloreactive ...) |
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| - | [[Image:1kj2.gif|left|200px]]<br /><applet load="1kj2" size=" | + | [[Image:1kj2.gif|left|200px]]<br /><applet load="1kj2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kj2, resolution 2.71Å" /> | caption="1kj2, resolution 2.71Å" /> | ||
'''Murine Alloreactive ScFv TCR-Peptide-MHC Class I Molecule Complex'''<br /> | '''Murine Alloreactive ScFv TCR-Peptide-MHC Class I Molecule Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The elongated complementary-determining region (CDR) 3beta found in the | + | The elongated complementary-determining region (CDR) 3beta found in the unliganded KB5-C20 TCR protrudes from the antigen binding site and prevents its docking onto the peptide/MHC (pMHC) surface according to a canonical diagonal orientation. We now present the crystal structure of a complex involving the KB5-C20 TCR and an octapeptide bound to the allogeneic H-2K(b) MHC class I molecule. This structure reveals how a tremendously large CDR3beta conformational change allows the KB5-C20 TCR to adapt to the rather constrained pMHC surface and achieve a diagonal docking mode. This extreme case of induced fit also shows that TCR plasticity is primarily restricted to CDR3 loops and does not propagate away from the antigen binding site. |
==About this Structure== | ==About this Structure== | ||
| - | 1KJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Darnault, C.]] | [[Category: Darnault, C.]] | ||
| - | [[Category: Fontecilla-Camps, J | + | [[Category: Fontecilla-Camps, J C.]] |
[[Category: Gregoire, C.]] | [[Category: Gregoire, C.]] | ||
[[Category: Guimezanes, A.]] | [[Category: Guimezanes, A.]] | ||
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[[Category: Mazza, G.]] | [[Category: Mazza, G.]] | ||
[[Category: Mosser, T.]] | [[Category: Mosser, T.]] | ||
| - | [[Category: Reiser, J | + | [[Category: Reiser, J B.]] |
| - | [[Category: Schmitt-Verhulst, A | + | [[Category: Schmitt-Verhulst, A M.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: allogeneic]] | [[Category: allogeneic]] | ||
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[[Category: tcr-pmhc complex]] | [[Category: tcr-pmhc complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:41 2008'' |
Revision as of 11:34, 21 February 2008
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Murine Alloreactive ScFv TCR-Peptide-MHC Class I Molecule Complex
Overview
The elongated complementary-determining region (CDR) 3beta found in the unliganded KB5-C20 TCR protrudes from the antigen binding site and prevents its docking onto the peptide/MHC (pMHC) surface according to a canonical diagonal orientation. We now present the crystal structure of a complex involving the KB5-C20 TCR and an octapeptide bound to the allogeneic H-2K(b) MHC class I molecule. This structure reveals how a tremendously large CDR3beta conformational change allows the KB5-C20 TCR to adapt to the rather constrained pMHC surface and achieve a diagonal docking mode. This extreme case of induced fit also shows that TCR plasticity is primarily restricted to CDR3 loops and does not propagate away from the antigen binding site.
About this Structure
1KJ2 is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
A T cell receptor CDR3beta loop undergoes conformational changes of unprecedented magnitude upon binding to a peptide/MHC class I complex., Reiser JB, Gregoire C, Darnault C, Mosser T, Guimezanes A, Schmitt-Verhulst AM, Fontecilla-Camps JC, Mazza G, Malissen B, Housset D, Immunity. 2002 Mar;16(3):345-54. PMID:11911820
Page seeded by OCA on Thu Feb 21 13:34:41 2008
