1koa
From Proteopedia
(New page: 200px<br /><applet load="1koa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1koa, resolution 3.3Å" /> '''TWITCHIN KINASE FRAGM...) |
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| - | [[Image:1koa.gif|left|200px]]<br /><applet load="1koa" size=" | + | [[Image:1koa.gif|left|200px]]<br /><applet load="1koa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1koa, resolution 3.3Å" /> | caption="1koa, resolution 3.3Å" /> | ||
'''TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS'''<br /> | '''TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The myosin-associated giant protein kinases twitchin and titin are | + | The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I. |
==About this Structure== | ==About this Structure== | ||
| - | 1KOA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http:// | + | 1KOA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Benian, G | + | [[Category: Benian, G M.]] |
| - | [[Category: Feil, S | + | [[Category: Feil, S C.]] |
[[Category: Heierhorst, J.]] | [[Category: Heierhorst, J.]] | ||
| - | [[Category: Kemp, B | + | [[Category: Kemp, B E.]] |
[[Category: Kobe, B.]] | [[Category: Kobe, B.]] | ||
| - | [[Category: Parker, M | + | [[Category: Parker, M W.]] |
| - | [[Category: Weiss, K | + | [[Category: Weiss, K R.]] |
[[Category: intrasteric regulation]] | [[Category: intrasteric regulation]] | ||
[[Category: kinase]] | [[Category: kinase]] | ||
[[Category: twitchin]] | [[Category: twitchin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:13 2008'' |
Revision as of 11:36, 21 February 2008
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TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS
Overview
The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I.
About this Structure
1KOA is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Giant protein kinases: domain interactions and structural basis of autoregulation., Kobe B, Heierhorst J, Feil SC, Parker MW, Benian GM, Weiss KR, Kemp BE, EMBO J. 1996 Dec 16;15(24):6810-21. PMID:9003756
Page seeded by OCA on Thu Feb 21 13:36:13 2008
