1knz
From Proteopedia
(New page: 200px<br /><applet load="1knz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1knz, resolution 2.45Å" /> '''Recognition of the r...) |
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| - | [[Image:1knz.gif|left|200px]]<br /><applet load="1knz" size=" | + | [[Image:1knz.gif|left|200px]]<br /><applet load="1knz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1knz, resolution 2.45Å" /> | caption="1knz, resolution 2.45Å" /> | ||
'''Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer'''<br /> | '''Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as | + | Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as nonstructural protein 3 (NSP3) for translation of viral mRNAs. NSP3 binds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G. The X-ray structure of the NSP3 RNA binding domain bound to a rotaviral mRNA 3' end has been determined. NSP3 is a novel, heart-shaped homodimer with a medial RNA binding cleft. The homodimer is asymmetric, and contains two similar N-terminal segments plus two structurally different C-terminal segments that intertwine to create a tunnel enveloping the mRNA 3' end. Biophysical studies demonstrate high affinity binding leading to increased thermal stability and slow dissociation kinetics, consistent with NSP3 function. |
==About this Structure== | ==About this Structure== | ||
| - | 1KNZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http:// | + | 1KNZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Simian rotavirus a/sa11]] | [[Category: Simian rotavirus a/sa11]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Deo, R | + | [[Category: Deo, R C.]] |
| - | [[Category: Groft, C | + | [[Category: Groft, C M.]] |
| - | [[Category: Rajashankar, K | + | [[Category: Rajashankar, K R.]] |
[[Category: protein-ssrna complex]] | [[Category: protein-ssrna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:11 2008'' |
Revision as of 11:36, 21 February 2008
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Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer
Overview
Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as nonstructural protein 3 (NSP3) for translation of viral mRNAs. NSP3 binds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G. The X-ray structure of the NSP3 RNA binding domain bound to a rotaviral mRNA 3' end has been determined. NSP3 is a novel, heart-shaped homodimer with a medial RNA binding cleft. The homodimer is asymmetric, and contains two similar N-terminal segments plus two structurally different C-terminal segments that intertwine to create a tunnel enveloping the mRNA 3' end. Biophysical studies demonstrate high affinity binding leading to increased thermal stability and slow dissociation kinetics, consistent with NSP3 function.
About this Structure
1KNZ is a Single protein structure of sequence from Simian rotavirus a/sa11. Full crystallographic information is available from OCA.
Reference
Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer., Deo RC, Groft CM, Rajashankar KR, Burley SK, Cell. 2002 Jan 11;108(1):71-81. PMID:11792322
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