1koo
From Proteopedia
(New page: 200px<br /> <applet load="1koo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1koo, resolution 3.8Å" /> '''THE CRYSTAL STRUCTUR...) |
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| - | [[Image:1koo.gif|left|200px]]<br /> | + | [[Image:1koo.gif|left|200px]]<br /><applet load="1koo" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1koo" size=" | + | |
caption="1koo, resolution 3.8Å" /> | caption="1koo, resolution 3.8Å" /> | ||
'''THE CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF A NOVEL RNA-BINDING DOMAIN FOUND IN THE HUMAN TAP NUCLEAR MRNA EXPORT FACTOR'''<br /> | '''THE CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF A NOVEL RNA-BINDING DOMAIN FOUND IN THE HUMAN TAP NUCLEAR MRNA EXPORT FACTOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Tap protein mediates the sequence nonspecific nuclear export of | + | The Tap protein mediates the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element (CTE). Previously, the structures of individual Tap subdomains, including ribonucleoprotein and leucine-rich repeat domains, have been described. Here, we report the crystal structure of a functional CTE RNA-binding domain of human Tap, including the N-terminal arm of the ribonucleoprotein domain and interdomain linking polypeptide. To identify residues that interact with the CTE, we have introduced 38 alanine substitutions for surface residues in the Tap CTE-binding domain and tested these mutants for their ability to support CTE-dependent nuclear RNA export and CTE binding. Four residues that cluster on a concave surface in the leucine-rich repeat domain were found to be critical for CTE binding and define a CTE-interacting surface on this domain. The second critical CTE-interacting surface on Tap is defined by three previously identified residues on the surface of the ribonucleoprotein domain. The structural and mutational data define a novel RNA-binding site on the Tap protein. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KOO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1KOO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Coburn, G | + | [[Category: Coburn, G A.]] |
| - | [[Category: Cullen, B | + | [[Category: Cullen, B R.]] |
| - | [[Category: Georgiadis, M | + | [[Category: Georgiadis, M M.]] |
| - | [[Category: Ho, D | + | [[Category: Ho, D N.]] |
[[Category: Kang, Y.]] | [[Category: Kang, Y.]] | ||
[[Category: constitutive transport element (cte)]] | [[Category: constitutive transport element (cte)]] | ||
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[[Category: ribonucleoprotein (rnp) and leucine rich repeat (lrr) domains]] | [[Category: ribonucleoprotein (rnp) and leucine rich repeat (lrr) domains]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:21 2008'' |
Revision as of 11:36, 21 February 2008
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THE CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF A NOVEL RNA-BINDING DOMAIN FOUND IN THE HUMAN TAP NUCLEAR MRNA EXPORT FACTOR
Contents |
Overview
The Tap protein mediates the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element (CTE). Previously, the structures of individual Tap subdomains, including ribonucleoprotein and leucine-rich repeat domains, have been described. Here, we report the crystal structure of a functional CTE RNA-binding domain of human Tap, including the N-terminal arm of the ribonucleoprotein domain and interdomain linking polypeptide. To identify residues that interact with the CTE, we have introduced 38 alanine substitutions for surface residues in the Tap CTE-binding domain and tested these mutants for their ability to support CTE-dependent nuclear RNA export and CTE binding. Four residues that cluster on a concave surface in the leucine-rich repeat domain were found to be critical for CTE binding and define a CTE-interacting surface on this domain. The second critical CTE-interacting surface on Tap is defined by three previously identified residues on the surface of the ribonucleoprotein domain. The structural and mutational data define a novel RNA-binding site on the Tap protein.
Disease
Known diseases associated with this structure: Bare lymphocyte syndrome, type I OMIM:[170260]
About this Structure
1KOO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure and mutational analysis of a novel RNA-binding domain found in the human Tap nuclear mRNA export factor., Ho DN, Coburn GA, Kang Y, Cullen BR, Georgiadis MM, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1888-93. PMID:11854490
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