1kp7
From Proteopedia
(New page: 200px<br /><applet load="1kp7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kp7" /> '''Conserved RNA Structure within the HCV IRES ...) |
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| - | [[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size=" | + | [[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Conserved RNA Structure within the HCV IRES eIF3 Binding Site'''<br /> | '''Conserved RNA Structure within the HCV IRES eIF3 Binding Site'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The hepatitis C virus (HCV) internal ribosome entry site (IRES) is | + | The hepatitis C virus (HCV) internal ribosome entry site (IRES) is recognized specifically by the small ribosomal subunit and eukaryotic initiation factor 3 (eIF3) before viral translation initiation. Using extensive mutagenesis and structure probing analysis, we show that the eIF3-binding domain of the HCV IRES contains an internal loop structure (loop IIIb) and an adjacent mismatched helix that are important for IRES-dependent initiation of translation. NMR studies reveal a unique three-dimensional structure for this internal loop that is conserved between viral isolates of varying primary sequence in this region. These data indicate that internal loop IIIb may be an attractive target for structure-based design of new antiviral agents. |
==About this Structure== | ==About this Structure== | ||
| - | 1KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KP7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Aboul-ela, F.]] | [[Category: Aboul-ela, F.]] | ||
| - | [[Category: Cole, P | + | [[Category: Cole, P T.]] |
| - | [[Category: Collier, A | + | [[Category: Collier, A J.]] |
[[Category: Gallego, J.]] | [[Category: Gallego, J.]] | ||
| - | [[Category: Harris, S | + | [[Category: Harris, S J.]] |
| - | [[Category: Harrison, G | + | [[Category: Harrison, G P.]] |
[[Category: Klinck, R.]] | [[Category: Klinck, R.]] | ||
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
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[[Category: s-turn]] | [[Category: s-turn]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:29 2008'' |
Revision as of 11:36, 21 February 2008
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Conserved RNA Structure within the HCV IRES eIF3 Binding Site
Overview
The hepatitis C virus (HCV) internal ribosome entry site (IRES) is recognized specifically by the small ribosomal subunit and eukaryotic initiation factor 3 (eIF3) before viral translation initiation. Using extensive mutagenesis and structure probing analysis, we show that the eIF3-binding domain of the HCV IRES contains an internal loop structure (loop IIIb) and an adjacent mismatched helix that are important for IRES-dependent initiation of translation. NMR studies reveal a unique three-dimensional structure for this internal loop that is conserved between viral isolates of varying primary sequence in this region. These data indicate that internal loop IIIb may be an attractive target for structure-based design of new antiviral agents.
About this Structure
1KP7 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A conserved RNA structure within the HCV IRES eIF3-binding site., Collier AJ, Gallego J, Klinck R, Cole PT, Harris SJ, Harrison GP, Aboul-Ela F, Varani G, Walker S, Nat Struct Biol. 2002 May;9(5):375-80. PMID:11927954
Page seeded by OCA on Thu Feb 21 13:36:29 2008
Categories: Protein complex | Aboul-ela, F. | Cole, P T. | Collier, A J. | Gallego, J. | Harris, S J. | Harrison, G P. | Klinck, R. | Varani, G. | Walker, S. | Cytosine mismatch | Eif3 | Hcv | Internal loop | Ires | S-turn
