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1kpz
From Proteopedia
(New page: 200px<br /><applet load="1kpz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kpz" /> '''PEMV-1 P1-P2 Frameshifting Pseudoknot Regula...) |
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| - | [[Image:1kpz.gif|left|200px]]<br /><applet load="1kpz" size=" | + | [[Image:1kpz.gif|left|200px]]<br /><applet load="1kpz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kpz" /> | caption="1kpz" /> | ||
'''PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure'''<br /> | '''PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A hairpin-type messenger RNA pseudoknot from pea enation mosaic virus RNA1 | + | A hairpin-type messenger RNA pseudoknot from pea enation mosaic virus RNA1 (PEMV-1) regulates the efficiency of programmed -1 ribosomal frameshifting. The solution structure and 15N relaxation rates reveal that the PEMV-1 pseudoknot is a compact-folded structure composed almost entirely of RNA triple helix. A three nucleotide reverse turn in loop 1 positions a protonated cytidine, C(10), in the correct orientation to form an A((n-1)).C(+).G-C(n) major groove base quadruple, like that found in the beet western yellows virus pseudoknot and the hepatitis delta virus ribozyme, despite distinct structural contexts. A novel loop 2-loop 1 A.U Hoogsteen base-pair stacks on the C(10)(+).G(28) base-pair of the A(12).C(10)(+).G(28)-C(13) quadruple and forms a wedge between the pseudoknot stems stabilizing a bent and over-rotated global conformation. Substitution of key nucleotides that stabilize the unique conformation of the PEMV-1 pseudoknot greatly reduces ribosomal frameshifting efficacy. |
==About this Structure== | ==About this Structure== | ||
| - | 1KPZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1KPZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPZ OCA]. |
==Reference== | ==Reference== | ||
Solution structure of a luteoviral P1-P2 frameshifting mRNA pseudoknot., Nixon PL, Rangan A, Kim YG, Rich A, Hoffman DW, Hennig M, Giedroc DP, J Mol Biol. 2002 Sep 20;322(3):621-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12225754 12225754] | Solution structure of a luteoviral P1-P2 frameshifting mRNA pseudoknot., Nixon PL, Rangan A, Kim YG, Rich A, Hoffman DW, Hennig M, Giedroc DP, J Mol Biol. 2002 Sep 20;322(3):621-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12225754 12225754] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Giedroc, D | + | [[Category: Giedroc, D P.]] |
| - | [[Category: Nixon, P | + | [[Category: Nixon, P L.]] |
[[Category: frameshifting]] | [[Category: frameshifting]] | ||
[[Category: luteovirus]] | [[Category: luteovirus]] | ||
| Line 22: | Line 22: | ||
[[Category: triple helix]] | [[Category: triple helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:46 2008'' |
Revision as of 11:36, 21 February 2008
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PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure
Overview
A hairpin-type messenger RNA pseudoknot from pea enation mosaic virus RNA1 (PEMV-1) regulates the efficiency of programmed -1 ribosomal frameshifting. The solution structure and 15N relaxation rates reveal that the PEMV-1 pseudoknot is a compact-folded structure composed almost entirely of RNA triple helix. A three nucleotide reverse turn in loop 1 positions a protonated cytidine, C(10), in the correct orientation to form an A((n-1)).C(+).G-C(n) major groove base quadruple, like that found in the beet western yellows virus pseudoknot and the hepatitis delta virus ribozyme, despite distinct structural contexts. A novel loop 2-loop 1 A.U Hoogsteen base-pair stacks on the C(10)(+).G(28) base-pair of the A(12).C(10)(+).G(28)-C(13) quadruple and forms a wedge between the pseudoknot stems stabilizing a bent and over-rotated global conformation. Substitution of key nucleotides that stabilize the unique conformation of the PEMV-1 pseudoknot greatly reduces ribosomal frameshifting efficacy.
About this Structure
1KPZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a luteoviral P1-P2 frameshifting mRNA pseudoknot., Nixon PL, Rangan A, Kim YG, Rich A, Hoffman DW, Hennig M, Giedroc DP, J Mol Biol. 2002 Sep 20;322(3):621-33. PMID:12225754
Page seeded by OCA on Thu Feb 21 13:36:46 2008
