1ku8
From Proteopedia
(New page: 200px<br /><applet load="1ku8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ku8, resolution 1.75Å" /> '''Crystal structure of...) |
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| - | [[Image:1ku8.gif|left|200px]]<br /><applet load="1ku8" size=" | + | [[Image:1ku8.gif|left|200px]]<br /><applet load="1ku8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ku8, resolution 1.75Å" /> | caption="1ku8, resolution 1.75Å" /> | ||
'''Crystal structure of Jacalin'''<br /> | '''Crystal structure of Jacalin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Evidence is presented that the specificity of jacalin, the seed lectin | + | Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin. |
==About this Structure== | ==About this Structure== | ||
| - | 1KU8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http:// | + | 1KU8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Artocarpus integer]] | [[Category: Artocarpus integer]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Astoul, C | + | [[Category: Astoul, C H.]] |
[[Category: Barre, A.]] | [[Category: Barre, A.]] | ||
[[Category: Bourne, Y.]] | [[Category: Bourne, Y.]] | ||
| - | [[Category: Damme, E | + | [[Category: Damme, E J.M Van.]] |
[[Category: Menu-Bouaouiche, L.]] | [[Category: Menu-Bouaouiche, L.]] | ||
| - | [[Category: Peumans, W | + | [[Category: Peumans, W J.]] |
[[Category: Rouge, P.]] | [[Category: Rouge, P.]] | ||
[[Category: Zamboni, V.]] | [[Category: Zamboni, V.]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:37:59 2008'' |
Revision as of 11:38, 21 February 2008
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Crystal structure of Jacalin
Overview
Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
About this Structure
1KU8 is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.
Reference
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090
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