1qnm
From Proteopedia
(New page: 200px<br /> <applet load="1qnm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qnm, resolution 2.3Å" /> '''HUMAN MANGANESE SUPE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1QNM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNM OCA]]. | + | 1QNM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]]. Structure known Active Site: MNA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Superoxide dismutase]] | ||
[[Category: Guan, Y.]] | [[Category: Guan, Y.]] | ||
[[Category: Tainer, J.A.]] | [[Category: Tainer, J.A.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:34:18 2007'' |
Revision as of 12:29, 30 October 2007
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HUMAN MANGANESE SUPEROXIDE DISMUTASE MUTANT Q143N
Overview
Structural and biochemical characterization of the nonliganding residue, glutamine 143 near the manganese of human Mn superoxide dismutase, (hMnSOD), a homotetramer of 22 kDa, reveals a functional role for this, residue. In the wild-type protein, the side-chain amide group of Gln 143, is about 5 A from the metal and is hydrogen-bonded to Tyr 34, which is a, second prominent side chain adjacent to the metal. We have prepared the, site-specific mutant of hMnSOD with the conservative replacement of Gln, 143 --> Asn (Q143N). The crystal structure of Q143N shows that the, side-chain amide nitrogen of residue 143 is 1.7 A more distant from the, manganese than in the wild-type enzyme. The Tyr 34 side-chain hydroxyl in, Q143N is also moved to become 0.6 A more distant from the metal due to an, ... [(full description)]
About this Structure
1QNM is a [Single protein] structure of sequence from [Homo sapiens] with MN as [ligand]. Active as [Superoxide dismutase], with EC number [1.15.1.1]. Structure known Active Site: MNA. Full crystallographic information is available from [OCA].
Reference
Probing the active site of human manganese superoxide dismutase: the role of glutamine 143., Hsieh Y, Guan Y, Tu C, Bratt PJ, Angerhofer A, Lepock JR, Hickey MJ, Tainer JA, Nick HS, Silverman DN, Biochemistry. 1998 Apr 7;37(14):4731-9. PMID:9537988
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