1kxk
From Proteopedia
(New page: 200px<br /><applet load="1kxk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kxk, resolution 3.0Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1kxk.gif|left|200px]]<br /><applet load="1kxk" size=" | + | [[Image:1kxk.gif|left|200px]]<br /><applet load="1kxk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kxk, resolution 3.0Å" /> | caption="1kxk, resolution 3.0Å" /> | ||
'''Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron'''<br /> | '''Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Group II self-splicing introns catalyze autoexcision from precursor RNA | + | Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6. |
==About this Structure== | ==About this Structure== | ||
| - | 1KXK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KXK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXK OCA]. |
==Reference== | ==Reference== | ||
Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11859154 11859154] | Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11859154 11859154] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Doudna, J | + | [[Category: Doudna, J A.]] |
[[Category: Zhang, L.]] | [[Category: Zhang, L.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 20: | Line 20: | ||
[[Category: two-nucleotide bulge]] | [[Category: two-nucleotide bulge]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:01 2008'' |
Revision as of 11:39, 21 February 2008
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Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron
Overview
Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6.
About this Structure
1KXK is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:11859154
Page seeded by OCA on Thu Feb 21 13:39:01 2008
