This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ky3
From Proteopedia
(New page: 200px<br /><applet load="1ky3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ky3, resolution 1.35Å" /> '''GDP-BOUND YPT7P AT 1...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ky3.jpg|left|200px]]<br /><applet load="1ky3" size=" | + | [[Image:1ky3.jpg|left|200px]]<br /><applet load="1ky3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ky3, resolution 1.35Å" /> | caption="1ky3, resolution 1.35Å" /> | ||
'''GDP-BOUND YPT7P AT 1.35 A RESOLUTION'''<br /> | '''GDP-BOUND YPT7P AT 1.35 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The GTPase Ypt7p from S. cerevisiae is involved in late | + | The GTPase Ypt7p from S. cerevisiae is involved in late endosome-to-vacuole transport and homotypic vacuole fusion. We present crystal structures of the GDP- and GppNHp-bound conformation of Ypt7p solved at 1.35 and 1.6 A resolution, respectively. Despite the similarity of the overall structure to other Ypt/Rab proteins, Ypt7p displays small but significant differences. The Ypt7p-specific residues Tyr33 and Tyr37 cause a difference in the main chain trace of the RabSF2 region and form a characteristic surface epitope. Ypt7p*GppNHp does not display the helix alpha2, characteristic of the Ras-superfamily, but instead possess an extended loop L4/L5. Due to insertions in loops L3 and L7, the neighboring RabSF1 and RabSF4 regions are different in their conformations to those of other Ypt/Rab proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1KY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1KY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KY3 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Constantinescu, A | + | [[Category: Constantinescu, A T.]] |
[[Category: Rak, A.]] | [[Category: Rak, A.]] | ||
| - | [[Category: Scheidig, A | + | [[Category: Scheidig, A J.]] |
[[Category: GDP]] | [[Category: GDP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 25: | Line 25: | ||
[[Category: ypt/rab protein]] | [[Category: ypt/rab protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:11 2008'' |
Revision as of 11:39, 21 February 2008
|
GDP-BOUND YPT7P AT 1.35 A RESOLUTION
Overview
The GTPase Ypt7p from S. cerevisiae is involved in late endosome-to-vacuole transport and homotypic vacuole fusion. We present crystal structures of the GDP- and GppNHp-bound conformation of Ypt7p solved at 1.35 and 1.6 A resolution, respectively. Despite the similarity of the overall structure to other Ypt/Rab proteins, Ypt7p displays small but significant differences. The Ypt7p-specific residues Tyr33 and Tyr37 cause a difference in the main chain trace of the RabSF2 region and form a characteristic surface epitope. Ypt7p*GppNHp does not display the helix alpha2, characteristic of the Ras-superfamily, but instead possess an extended loop L4/L5. Due to insertions in loops L3 and L7, the neighboring RabSF1 and RabSF4 regions are different in their conformations to those of other Ypt/Rab proteins.
About this Structure
1KY3 is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Full crystallographic information is available from OCA.
Reference
Rab-subfamily-specific regions of Ypt7p are structurally different from other RabGTPases., Constantinescu AT, Rak A, Alexandrov K, Esters H, Goody RS, Scheidig AJ, Structure. 2002 Apr;10(4):569-79. PMID:11937061
Page seeded by OCA on Thu Feb 21 13:39:11 2008
