1ayx
From Proteopedia
(New page: 200px<br /> <applet load="1ayx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ayx, resolution 1.70Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AYX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomycopsis_fibuligera Saccharomycopsis fibuligera]] with TRS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AYX OCA]]. | + | 1AYX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomycopsis_fibuligera Saccharomycopsis fibuligera]] with TRS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3]]. Structure known Active Site: NUL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AYX OCA]]. |
==Reference== | ==Reference== | ||
Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution., Sevcik J, Solovicova A, Hostinova E, Gasperik J, Wilson KS, Dauter Z, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):854-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9757101 9757101] | Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution., Sevcik J, Solovicova A, Hostinova E, Gasperik J, Wilson KS, Dauter Z, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):854-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9757101 9757101] | ||
| + | [[Category: Glucan 1,4-alpha-glucosidase]] | ||
[[Category: Saccharomycopsis fibuligera]] | [[Category: Saccharomycopsis fibuligera]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: polysaccharide degradation]] | [[Category: polysaccharide degradation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:34:44 2007'' |
Revision as of 12:30, 30 October 2007
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CRYSTAL STRUCTURE OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS FIBULIGERA AT 1.7 ANGSTROMS
Overview
The yeast Saccharomycopsis fibuligera produces a glucoamylase which, belongs to sequence family 15 of glycosyl hydrolases. The structure of the, non-glycosyl-ated recombinant enzyme has been determined by molecular, replacement and refined against 1.7 A resolution synchrotron data to an R, factor of 14.6%. This is the first report of the three-dimensional, structure of a yeast family 15 glucoamylase. The refinement from the, initial molecular-replacement model was not straightforward. It involved, the use of an unrestrained automated refinement procedure (uARP) in, combination with the maximum-likelihood refinement program REFMAC. The, enzyme consists of 492 amino-acid residues and has 14 alpha-helices, 12 of, which form an (alpha/alpha)6 barrel. It contains a single catalytic domain, but ... [(full description)]
About this Structure
1AYX is a [Single protein] structure of sequence from [Saccharomycopsis fibuligera] with TRS as [ligand]. Active as [Glucan 1,4-alpha-glucosidase], with EC number [3.2.1.3]. Structure known Active Site: NUL. Full crystallographic information is available from [OCA].
Reference
Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution., Sevcik J, Solovicova A, Hostinova E, Gasperik J, Wilson KS, Dauter Z, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):854-66. PMID:9757101
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