1l0n
From Proteopedia
(New page: 200px<br /><applet load="1l0n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l0n, resolution 2.60Å" /> '''native structure of ...) |
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| - | [[Image:1l0n.gif|left|200px]]<br /><applet load="1l0n" size=" | + | [[Image:1l0n.gif|left|200px]]<br /><applet load="1l0n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l0n, resolution 2.60Å" /> | caption="1l0n, resolution 2.60Å" /> | ||
'''native structure of bovine mitochondrial cytochrome bc1 complex'''<br /> | '''native structure of bovine mitochondrial cytochrome bc1 complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ubiquinol cytochrome c oxido-reductase (EC. 1.10.2.2, bc1) is an integral | + | Ubiquinol cytochrome c oxido-reductase (EC. 1.10.2.2, bc1) is an integral membrane protein complex essential to cellular respiration. Structures of the 11-subunit mitochondrial bc1 complex were determined with and without the fungicide famoxadone. Specific inhibition by famoxadone is achieved through a coordinated optimization of aromatic-aromatic interactions where conformational rearrangements in famoxadone and in residues lining the inhibitor-binding pocket produce a network of aromatic-aromatic interactions that mimic the crystal lattice of benzene. The profound aromatic-aromatic interactions as supported by prior mutagenesis provide a structural basis for specific protein-ligand interaction in a hydrophobic environment. Dramatic conformational changes, both in cyt. b and ISP subunits in the inhibitor-protein complex, confer experimental evidence for a functional role of cytochrome b in the induced conformational arrest of ISP and allow the identification of a possible intrasubunit signal transduction pathway that controls the movement of ISP. These results support an inhibitory mechanism that is consistent with the requirement for ISP movement in the electron transfer of this complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1L0N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with HEM and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] Full crystallographic information is available from [http:// | + | 1L0N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Wen, X.]] | [[Category: Wen, X.]] | ||
[[Category: Xia, D.]] | [[Category: Xia, D.]] | ||
| - | [[Category: Yu, C | + | [[Category: Yu, C A.]] |
[[Category: Yu, L.]] | [[Category: Yu, L.]] | ||
[[Category: Zhang, L.]] | [[Category: Zhang, L.]] | ||
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[[Category: mpp]] | [[Category: mpp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:59 2008'' |
Revision as of 11:40, 21 February 2008
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native structure of bovine mitochondrial cytochrome bc1 complex
Overview
Ubiquinol cytochrome c oxido-reductase (EC. 1.10.2.2, bc1) is an integral membrane protein complex essential to cellular respiration. Structures of the 11-subunit mitochondrial bc1 complex were determined with and without the fungicide famoxadone. Specific inhibition by famoxadone is achieved through a coordinated optimization of aromatic-aromatic interactions where conformational rearrangements in famoxadone and in residues lining the inhibitor-binding pocket produce a network of aromatic-aromatic interactions that mimic the crystal lattice of benzene. The profound aromatic-aromatic interactions as supported by prior mutagenesis provide a structural basis for specific protein-ligand interaction in a hydrophobic environment. Dramatic conformational changes, both in cyt. b and ISP subunits in the inhibitor-protein complex, confer experimental evidence for a functional role of cytochrome b in the induced conformational arrest of ISP and allow the identification of a possible intrasubunit signal transduction pathway that controls the movement of ISP. These results support an inhibitory mechanism that is consistent with the requirement for ISP movement in the electron transfer of this complex.
About this Structure
1L0N is a Protein complex structure of sequences from Bos taurus with and as ligands. Active as Ubiquinol--cytochrome-c reductase, with EC number 1.10.2.2 Full crystallographic information is available from OCA.
Reference
The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition., Gao X, Wen X, Yu C, Esser L, Tsao S, Quinn B, Zhang L, Yu L, Xia D, Biochemistry. 2002 Oct 1;41(39):11692-702. PMID:12269811
Page seeded by OCA on Thu Feb 21 13:39:59 2008
Categories: Bos taurus | Protein complex | Ubiquinol--cytochrome-c reductase | Esser, L. | Gao, X. | Quinn, B. | Tsao, S. | Wen, X. | Xia, D. | Yu, C A. | Yu, L. | Zhang, L. | FES | HEM | Cytochrome b | Cytochrome bc1 | Cytochrome c1 | Heme protein | Iron sulfur protein | Membrane protein | Mitochondrial processing protease | Mpp
