1kr5
From Proteopedia
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(New page: 200px<br /> <applet load="1kr5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kr5, resolution 2.10Å" /> '''Crystal structure o...) |
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caption="1kr5, resolution 2.10Å" /> | caption="1kr5, resolution 2.10Å" /> | ||
'''Crystal structure of human L-isoaspartyl methyltransferase'''<br /> | '''Crystal structure of human L-isoaspartyl methyltransferase'''<br /> | ||
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| - | ==Overview== | ||
| - | The enzyme l-isoaspartyl methyltransferase initiates the repair of damaged, proteins by recognizing and methylating isomerized and racemized aspartyl, residues in aging proteins. The crystal structure of the human enzyme, containing a bound S-adenosyl-l-homocysteine cofactor is reported here at, a resolution of 2.1 A. A comparison of the human enzyme to homologs from, two other species reveals several significant differences among otherwise, similar structures. In all three structures, we find that three conserved, charged residues are buried in the protein interior near the active site., Electrostatics calculations suggest that these buried charges might make, significant contributions to the energetics of binding the charged, S-adenosyl-l-methionine cofactor and to catalysis. We suggest a possible, structural explanation for the observed differences in reactivity toward, the structurally similar l-isoaspartyl and d-aspartyl residues in the, human, archael, and eubacterial enzymes. Finally, the human structure, reveals that the known genetic polymorphism at residue 119 (Val/Ile) maps, to an exposed region away from the active site. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1KR5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-L-isoaspartate(D-aspartate)_O-methyltransferase Protein-L-isoaspartate(D-aspartate) O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.77 2.1.1.77] Full crystallographic information is available from [http:// | + | 1KR5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-L-isoaspartate(D-aspartate)_O-methyltransferase Protein-L-isoaspartate(D-aspartate) O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.77 2.1.1.77] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KR5 OCA]. |
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein-L-isoaspartate(D-aspartate) O-methyltransferase]] | [[Category: Protein-L-isoaspartate(D-aspartate) O-methyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Clarke, S.]] | [[Category: Clarke, S.]] | ||
| - | [[Category: Griffith, S | + | [[Category: Griffith, S C.]] |
| - | [[Category: MacLaren, D | + | [[Category: MacLaren, D C.]] |
[[Category: Ryttersgaard, C.]] | [[Category: Ryttersgaard, C.]] | ||
| - | [[Category: Sawaya, M | + | [[Category: Sawaya, M R.]] |
| - | [[Category: Yeates, T | + | [[Category: Yeates, T O.]] |
[[Category: SAH]] | [[Category: SAH]] | ||
[[Category: rossmann-fold doubly-wound-alpha-beta-alpha-sandwich]] | [[Category: rossmann-fold doubly-wound-alpha-beta-alpha-sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:03 2008'' |
Revision as of 11:40, 21 February 2008
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Crystal structure of human L-isoaspartyl methyltransferase
About this Structure
1KR5 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Protein-L-isoaspartate(D-aspartate) O-methyltransferase, with EC number 2.1.1.77 Full crystallographic information is available from OCA.
Page seeded by OCA on Thu Feb 21 13:40:03 2008
