1l0z

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(New page: 200px<br /><applet load="1l0z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l0z, resolution 1.5&Aring;" /> '''THE STRUCTURE OF PORC...)
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[[Image:1l0z.gif|left|200px]]<br /><applet load="1l0z" size="350" color="white" frame="true" align="right" spinBox="true"
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caption="1l0z, resolution 1.5&Aring;" />
'''THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL'''<br />
'''THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL'''<br />
==Overview==
==Overview==
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Crystals of porcine pancreatic elastase can be derived by soaking in, high-molarity bromide solutions. These crystals, either in a, glycerol-based cryoprotectant solution or in paraffin oil, can be, subsequently pressurized under a xenon atmosphere to incorporate xenon., When paraffin oil is used, a larger number of bromide ions are observed on, the protein surface. Intensity data collected to lower energy than the, bromine absorption edge can be used to determine the xenon position and, the resultant phase information can be used to determine the bromine, substructure from data collected to higher energy than the bromine, absorption edge. The method would appear to have general applicability, where large substructures need to be determined.
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Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.
==About this Structure==
==About this Structure==
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1L0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with NA, BR and XE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L0Z OCA].
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1L0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=XE:'>XE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0Z OCA].
==Reference==
==Reference==
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Panjikar, S.]]
[[Category: Panjikar, S.]]
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[[Category: Tucker, P.A.]]
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[[Category: Tucker, P A.]]
[[Category: BR]]
[[Category: BR]]
[[Category: NA]]
[[Category: NA]]
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[[Category: xenon]]
[[Category: xenon]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:09:04 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:06 2008''

Revision as of 11:40, 21 February 2008

Image:1l0z.gif

1l0z, resolution 1.5Å

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THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL

Overview

Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.

About this Structure

1L0Z is a Single protein structure of sequence from Sus scrofa with , and as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.

Reference

Xenon derivatization of halide-soaked protein crystals., Panjikar S, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1413-20. Epub 2002, Aug 23. PMID:12198297

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