1ksi
From Proteopedia
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caption="1ksi, resolution 2.2Å" /> | caption="1ksi, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION'''<br /> | ||
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- | ==Overview== | ||
- | BACKGROUND: Copper-containing amine oxidases catalyze the oxidative, deamination of primary amines to aldehydes, in a reaction that requires, free radicals. These enzymes are important in many biological processes, including cell differentiation and growth, would healing, detoxification, and signalling. The catalytic reaction requires a redox cofactor, topa, quinone (TPQ), which is derived by post-translational modification of an, invariant tyrosine residue. Both the biogenesis of the TPQ cofactor and, the reaction catalyzed by the enzyme require the presence of a copper atom, at the active site. The crystal structure of a prokaryotic copper amine, oxidase from E. coli (ECAO) has recently been reported. RESULTS: The first, structure of a eukaryotic (pea seedling) amine oxidase (PSAO) has been, solved and refined at 2.2 A resolution. The crystallographic phases were, derived from a single phosphotungstic acid derivative. The positions of, the tungsten atoms in the W12 clusters were obtained by molecular, replacement using E. coli amine oxidase as a search model. The methodology, avoided bias from the search model, and provides an essentially, independent view of a eukaryotic amine oxidase. The PSAO molecule is a, homodimer; each subunit has three domains. The active site of each subunit, lies near an edge of the beta-sandwich of the largest domain, but is not, accessible from the solvent. The essential active-site copper atom is, coordinated by three histidine side chains and two water molecules in an, approximately square-pyramidal arrangement. All the atoms of the TPQ, cofactor are unambiguously defined, the shortest distance to the copper, atom being approximately 6 A. CONCLUSIONS: There is considerable, structural homology between PSAO and ECAO. A combination of evidence from, both structures indicates that the TPQ side chain is sufficiently flexible, to permit the aromatic grouf to rotate about the Cbeta-Cgamma bond, and to, move between bonding and non-bonding positions with respect to the Cu, atom. Conformational flexibility is also required at the surface of the, molecule to allow the substrates access to the active site, which is, inaccessible to solvent, as expected for an enzyme that uses radical, chemistry. | ||
==About this Structure== | ==About this Structure== | ||
1KSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pivum_sativum Pivum sativum] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Known structural/functional Sites: <scene name='pdbsite=ACA:The+Cu+Atom+In+The+Active+Site+Of+Each+Subunit+Is+Ligate+...'>ACA</scene> and <scene name='pdbsite=ACB:The+Cu+Atom+In+The+Active+Site+Of+Each+Subunit+Is+Ligate+...'>ACB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSI OCA]. | 1KSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pivum_sativum Pivum sativum] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Known structural/functional Sites: <scene name='pdbsite=ACA:The+Cu+Atom+In+The+Active+Site+Of+Each+Subunit+Is+Ligate+...'>ACA</scene> and <scene name='pdbsite=ACB:The+Cu+Atom+In+The+Active+Site+Of+Each+Subunit+Is+Ligate+...'>ACB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSI OCA]. | ||
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- | ==Reference== | ||
- | Crystal structure of a eukaryotic (pea seedling) copper-containing amine oxidase at 2.2 A resolution., Kumar V, Dooley DM, Freeman HC, Guss JM, Harvey I, McGuirl MA, Wilce MC, Zubak VM, Structure. 1996 Aug 15;4(8):943-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805580 8805580] | ||
[[Category: Amine oxidase (copper-containing)]] | [[Category: Amine oxidase (copper-containing)]] | ||
[[Category: Pivum sativum]] | [[Category: Pivum sativum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
- | [[Category: Freeman, H | + | [[Category: Freeman, H C.]] |
- | [[Category: Guss, J | + | [[Category: Guss, J M.]] |
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
- | [[Category: Wilce, M | + | [[Category: Wilce, M C.J.]] |
[[Category: CU]] | [[Category: CU]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: pea seedling]] | [[Category: pea seedling]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:27 2008'' |
Revision as of 11:40, 21 February 2008
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CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION
About this Structure
1KSI is a Single protein structure of sequence from Pivum sativum with , and as ligands. Active as Amine oxidase (copper-containing), with EC number 1.4.3.6 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Page seeded by OCA on Thu Feb 21 13:40:27 2008
Categories: Amine oxidase (copper-containing) | Pivum sativum | Single protein | Freeman, H C. | Guss, J M. | Kumar, V. | Wilce, M C.J. | CU | MN | NAG | Copper | Oxidase | Oxidoreductase | Pea seedling