1l2q
From Proteopedia
(New page: 200px<br /><applet load="1l2q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2q, resolution 1.70Å" /> '''Crystal Structure of...) |
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| - | [[Image:1l2q.jpg|left|200px]]<br /><applet load="1l2q" size=" | + | [[Image:1l2q.jpg|left|200px]]<br /><applet load="1l2q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l2q, resolution 1.70Å" /> | caption="1l2q, resolution 1.70Å" /> | ||
'''Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)'''<br /> | '''Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Genes encoding methanogenic methylamine methyltransferases all contain an | + | Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. |
==About this Structure== | ==About this Structure== | ||
| - | 1L2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with NH4, X7O and BGX as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1L2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with <scene name='pdbligand=NH4:'>NH4</scene>, <scene name='pdbligand=X7O:'>X7O</scene> and <scene name='pdbligand=BGX:'>BGX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanosarcina barkeri]] | [[Category: Methanosarcina barkeri]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chan, M | + | [[Category: Chan, M K.]] |
| - | [[Category: Ferguson, T | + | [[Category: Ferguson, T K.]] |
[[Category: Gong, W.]] | [[Category: Gong, W.]] | ||
[[Category: Hao, B.]] | [[Category: Hao, B.]] | ||
| - | [[Category: James, C | + | [[Category: James, C M.]] |
| - | [[Category: Krzycki, J | + | [[Category: Krzycki, J A.]] |
[[Category: BGX]] | [[Category: BGX]] | ||
[[Category: NH4]] | [[Category: NH4]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:42 2008'' |
Revision as of 11:40, 21 February 2008
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Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)
Overview
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.
About this Structure
1L2Q is a Single protein structure of sequence from Methanosarcina barkeri with , and as ligands. Full crystallographic information is available from OCA.
Reference
A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:12029132
Page seeded by OCA on Thu Feb 21 13:40:42 2008
Categories: Methanosarcina barkeri | Single protein | Chan, M K. | Ferguson, T K. | Gong, W. | Hao, B. | James, C M. | Krzycki, J A. | BGX | NH4 | X7O | Tim barrel
