This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1l2q
From Proteopedia
(New page: 200px<br /><applet load="1l2q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2q, resolution 1.70Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1l2q.jpg|left|200px]]<br /><applet load="1l2q" size=" | + | [[Image:1l2q.jpg|left|200px]]<br /><applet load="1l2q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l2q, resolution 1.70Å" /> | caption="1l2q, resolution 1.70Å" /> | ||
'''Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)'''<br /> | '''Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Genes encoding methanogenic methylamine methyltransferases all contain an | + | Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. |
==About this Structure== | ==About this Structure== | ||
| - | 1L2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with NH4, X7O and BGX as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1L2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with <scene name='pdbligand=NH4:'>NH4</scene>, <scene name='pdbligand=X7O:'>X7O</scene> and <scene name='pdbligand=BGX:'>BGX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2Q OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Methanosarcina barkeri]] | [[Category: Methanosarcina barkeri]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chan, M | + | [[Category: Chan, M K.]] |
| - | [[Category: Ferguson, T | + | [[Category: Ferguson, T K.]] |
[[Category: Gong, W.]] | [[Category: Gong, W.]] | ||
[[Category: Hao, B.]] | [[Category: Hao, B.]] | ||
| - | [[Category: James, C | + | [[Category: James, C M.]] |
| - | [[Category: Krzycki, J | + | [[Category: Krzycki, J A.]] |
[[Category: BGX]] | [[Category: BGX]] | ||
[[Category: NH4]] | [[Category: NH4]] | ||
| Line 24: | Line 24: | ||
[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:42 2008'' |
Revision as of 11:40, 21 February 2008
|
Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)
Overview
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.
About this Structure
1L2Q is a Single protein structure of sequence from Methanosarcina barkeri with , and as ligands. Full crystallographic information is available from OCA.
Reference
A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:12029132
Page seeded by OCA on Thu Feb 21 13:40:42 2008
Categories: Methanosarcina barkeri | Single protein | Chan, M K. | Ferguson, T K. | Gong, W. | Hao, B. | James, C M. | Krzycki, J A. | BGX | NH4 | X7O | Tim barrel
