1l3a
From Proteopedia
(New page: 200px<br /><applet load="1l3a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3a, resolution 2.30Å" /> '''Structure of the pla...) |
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| - | [[Image:1l3a.jpg|left|200px]]<br /><applet load="1l3a" size=" | + | [[Image:1l3a.jpg|left|200px]]<br /><applet load="1l3a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l3a, resolution 2.30Å" /> | caption="1l3a, resolution 2.30Å" /> | ||
'''Structure of the plant transcriptional regulator PBF-2'''<br /> | '''Structure of the plant transcriptional regulator PBF-2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of p24, the single-stranded DNA (ssDNA) binding | + | The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression. |
==About this Structure== | ==About this Structure== | ||
| - | 1L3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http:// | + | 1L3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: whirly]] | [[Category: whirly]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:53 2008'' |
Revision as of 11:40, 21 February 2008
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Structure of the plant transcriptional regulator PBF-2
Overview
The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.
About this Structure
1L3A is a Single protein structure of sequence from Solanum tuberosum. Full crystallographic information is available from OCA.
Reference
A new family of plant transcription factors displays a novel ssDNA-binding surface., Desveaux D, Allard J, Brisson N, Sygusch J, Nat Struct Biol. 2002 Jul;9(7):512-7. PMID:12080340
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