1l3x
From Proteopedia
(New page: 200px<br /><applet load="1l3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3x" /> '''Solution Structure of Novel Disintegrin Salm...) |
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| - | [[Image:1l3x.gif|left|200px]]<br /><applet load="1l3x" size=" | + | [[Image:1l3x.gif|left|200px]]<br /><applet load="1l3x" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of Novel Disintegrin Salmosin'''<br /> | '''Solution Structure of Novel Disintegrin Salmosin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Disintegrins are potent inhibitors of both platelet aggregation and | + | Disintegrins are potent inhibitors of both platelet aggregation and integrin-dependent cell adhesion. A new disintegrin, salmosin, isolated from the venom of the Korean snake Agkistrodon halys brevicaudus, has been characterized by mass spectrometry and NMR spectroscopy, and its in vitro biological activity has been assessed. The IC(50) value of the purified salmosin was determined to be 2.2 nM in an assay for the inhibition of glycoprotein IIb-IIIa/fibrinogen interaction. Salmosin also inhibited the bovine capillary endothelial cell proliferation induced by bFGF in a dose-dependent manner. The NMR solution structures were well converged with a root-mean-square deviation of 0.76 A for backbone atoms among the 20 lowest energy structures, except for the arginylglycylaspartic acid (RGD) loop. The structure revealed that the conserved RGD motif with an unusual finger shape is distal from the rigid core of the C-terminal domain. Furthermore, even though the RGD motif did not interact with the hydrophobic core of the protein, it was stabilized by a network of molecular contacts through a small antiparallel beta-sheet comprising residues of Ile46-Ala50 and Asp54-Tyr58. Last, the electrostatic charge distribution on the surface of salmosin differs dramatically from that of other disintegrin proteins in that there is a cluster of negatively charged residues in close proximity to the RGD loop. |
==About this Structure== | ==About this Structure== | ||
| - | 1L3X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gloydius_blomhoffi_brevicaudus Gloydius blomhoffi brevicaudus]. Full crystallographic information is available from [http:// | + | 1L3X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gloydius_blomhoffi_brevicaudus Gloydius blomhoffi brevicaudus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: snake venome]] | [[Category: snake venome]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:02 2008'' |
Revision as of 11:41, 21 February 2008
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Solution Structure of Novel Disintegrin Salmosin
Overview
Disintegrins are potent inhibitors of both platelet aggregation and integrin-dependent cell adhesion. A new disintegrin, salmosin, isolated from the venom of the Korean snake Agkistrodon halys brevicaudus, has been characterized by mass spectrometry and NMR spectroscopy, and its in vitro biological activity has been assessed. The IC(50) value of the purified salmosin was determined to be 2.2 nM in an assay for the inhibition of glycoprotein IIb-IIIa/fibrinogen interaction. Salmosin also inhibited the bovine capillary endothelial cell proliferation induced by bFGF in a dose-dependent manner. The NMR solution structures were well converged with a root-mean-square deviation of 0.76 A for backbone atoms among the 20 lowest energy structures, except for the arginylglycylaspartic acid (RGD) loop. The structure revealed that the conserved RGD motif with an unusual finger shape is distal from the rigid core of the C-terminal domain. Furthermore, even though the RGD motif did not interact with the hydrophobic core of the protein, it was stabilized by a network of molecular contacts through a small antiparallel beta-sheet comprising residues of Ile46-Ala50 and Asp54-Tyr58. Last, the electrostatic charge distribution on the surface of salmosin differs dramatically from that of other disintegrin proteins in that there is a cluster of negatively charged residues in close proximity to the RGD loop.
About this Structure
1L3X is a Single protein structure of sequence from Gloydius blomhoffi brevicaudus. Full crystallographic information is available from OCA.
Reference
Solution structure of a novel disintegrin, salmosin, from Agkistrondon halys venom., Shin J, Hong SY, Chung K, Kang I, Jang Y, Kim DS, Lee W, Biochemistry. 2003 Dec 16;42(49):14408-15. PMID:14661951
Page seeded by OCA on Thu Feb 21 13:41:02 2008
