1l3z

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(New page: 200px<br /><applet load="1l3z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3z, resolution 2.01&Aring;" /> '''Crystal Structure An...)
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caption="1l3z, resolution 2.01&Aring;" />
'''Crystal Structure Analysis of an RNA Heptamer'''<br />
'''Crystal Structure Analysis of an RNA Heptamer'''<br />
==Overview==
==Overview==
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The crystal structure of an alternating RNA heptamer r(GUAUACA) has been, determined to 2.0 A resolution and refined to an R(work) of 17.1% and, R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the, space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A, and two independent strands in the asymmetric unit. Each heptamer forms a, duplex with its symmetry-related strand and each duplex contains six, Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two, kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on, each other forming a pseudo-continuous column, which is typical of the RNA, packing mode, while duplex 2 is typical of A-DNA packing with its termini, in abutting interactions. Overhang adenine residues stack within the, duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes, 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged, to two N1 atoms of symmetry-related A7 bases.
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The crystal structure of an alternating RNA heptamer r(GUAUACA) has been determined to 2.0 A resolution and refined to an R(work) of 17.1% and R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A and two independent strands in the asymmetric unit. Each heptamer forms a duplex with its symmetry-related strand and each duplex contains six Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on each other forming a pseudo-continuous column, which is typical of the RNA packing mode, while duplex 2 is typical of A-DNA packing with its termini in abutting interactions. Overhang adenine residues stack within the duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged to two N1 atoms of symmetry-related A7 bases.
==About this Structure==
==About this Structure==
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1L3Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L3Z OCA].
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1L3Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3Z OCA].
==Reference==
==Reference==
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[[Category: rna]]
[[Category: rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:28:09 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:04 2008''

Revision as of 11:41, 21 February 2008

Image:1l3z.gif

1l3z, resolution 2.01Å

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Crystal Structure Analysis of an RNA Heptamer

Overview

The crystal structure of an alternating RNA heptamer r(GUAUACA) has been determined to 2.0 A resolution and refined to an R(work) of 17.1% and R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A and two independent strands in the asymmetric unit. Each heptamer forms a duplex with its symmetry-related strand and each duplex contains six Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on each other forming a pseudo-continuous column, which is typical of the RNA packing mode, while duplex 2 is typical of A-DNA packing with its termini in abutting interactions. Overhang adenine residues stack within the duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged to two N1 atoms of symmetry-related A7 bases.

About this Structure

1L3Z is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

The crystal structure of an alternating RNA heptamer r(GUAUACA) forming a six base-paired duplex with 3'-end adenine overhangs., Shi K, Pan B, Sundaralingam M, Nucleic Acids Res. 2003 Mar 1;31(5):1392-7. PMID:12595546

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