1l9u
From Proteopedia
(New page: 200px<br /><applet load="1l9u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l9u, resolution 4.0Å" /> '''THERMUS AQUATICUS RNA...) |
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| - | [[Image:1l9u.gif|left|200px]]<br /><applet load="1l9u" size=" | + | [[Image:1l9u.gif|left|200px]]<br /><applet load="1l9u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l9u, resolution 4.0Å" /> | caption="1l9u, resolution 4.0Å" /> | ||
'''THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION'''<br /> | '''THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the initiating form of Thermus aquaticus RNA | + | The crystal structure of the initiating form of Thermus aquaticus RNA polymerase, containing core RNA polymerase (alpha2betabeta'omega) and the promoter specificity sigma subunit, has been determined at 4 angstrom resolution. Important structural features of the RNA polymerase and their roles in positioning sigma within the initiation complex are delineated, as well as the role played by sigma in modulating the opening of the RNA polymerase active-site channel. The two carboxyl-terminal domains of sigma are separated by 45 angstroms on the surface of the RNA polymerase, but are linked by an extended loop. The loop winds near the RNA polymerase active site, where it may play a role in initiating nucleotide substrate binding, and out through the RNA exit channel. The advancing RNA transcript must displace the loop, leading to abortive initiation and ultimately to sigma release. |
==About this Structure== | ==About this Structure== | ||
| - | 1L9U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with MG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 1L9U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L9U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
| - | [[Category: Darst, S | + | [[Category: Darst, S A.]] |
[[Category: Masuda, S.]] | [[Category: Masuda, S.]] | ||
| - | [[Category: Murakami, K | + | [[Category: Murakami, K S.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:42:55 2008'' |
Revision as of 11:42, 21 February 2008
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THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION
Overview
The crystal structure of the initiating form of Thermus aquaticus RNA polymerase, containing core RNA polymerase (alpha2betabeta'omega) and the promoter specificity sigma subunit, has been determined at 4 angstrom resolution. Important structural features of the RNA polymerase and their roles in positioning sigma within the initiation complex are delineated, as well as the role played by sigma in modulating the opening of the RNA polymerase active-site channel. The two carboxyl-terminal domains of sigma are separated by 45 angstroms on the surface of the RNA polymerase, but are linked by an extended loop. The loop winds near the RNA polymerase active site, where it may play a role in initiating nucleotide substrate binding, and out through the RNA exit channel. The advancing RNA transcript must displace the loop, leading to abortive initiation and ultimately to sigma release.
About this Structure
1L9U is a Protein complex structure of sequences from Thermus aquaticus with and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution., Murakami KS, Masuda S, Darst SA, Science. 2002 May 17;296(5571):1280-4. PMID:12016306
Page seeded by OCA on Thu Feb 21 13:42:55 2008
