1lb2
From Proteopedia
(New page: 200px<br /> <applet load="1lb2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lb2, resolution 3.1Å" /> '''Structure of the E. ...) |
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| - | [[Image:1lb2.gif|left|200px]]<br /> | + | [[Image:1lb2.gif|left|200px]]<br /><applet load="1lb2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lb2" size=" | + | |
caption="1lb2, resolution 3.1Å" /> | caption="1lb2, resolution 3.1Å" /> | ||
'''Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA'''<br /> | '''Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Escherichia coli catabolite activator protein (CAP) activates | + | The Escherichia coli catabolite activator protein (CAP) activates transcription at P(lac), P(gal), and other promoters through interactions with the RNA polymerase alpha subunit carboxyl-terminal domain (alphaCTD). We determined the crystal structure of the CAP-alphaCTD-DNA complex at a resolution of 3.1 angstroms. CAP makes direct protein-protein interactions with alphaCTD, and alphaCTD makes direct protein-DNA interactions with the DNA segment adjacent to the DNA site for CAP. There are no large-scale conformational changes in CAP and alphaCTD, and the interface between CAP and alphaCTD is small. These findings are consistent with the proposal that activation involves a simple "recruitment" mechanism. |
==About this Structure== | ==About this Structure== | ||
| - | 1LB2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LB2 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 1LB2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LB2 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Benoff, B.]] | [[Category: Benoff, B.]] | ||
| - | [[Category: Berman, H | + | [[Category: Berman, H M.]] |
[[Category: Blatter, E.]] | [[Category: Blatter, E.]] | ||
| - | [[Category: Ebright, R | + | [[Category: Ebright, R H.]] |
| - | [[Category: Ebright, Y | + | [[Category: Ebright, Y W.]] |
| - | [[Category: Lawson, C | + | [[Category: Lawson, C L.]] |
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Parkinson, G.]] | [[Category: Parkinson, G.]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:15 2008'' |
Revision as of 11:43, 21 February 2008
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Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA
Overview
The Escherichia coli catabolite activator protein (CAP) activates transcription at P(lac), P(gal), and other promoters through interactions with the RNA polymerase alpha subunit carboxyl-terminal domain (alphaCTD). We determined the crystal structure of the CAP-alphaCTD-DNA complex at a resolution of 3.1 angstroms. CAP makes direct protein-protein interactions with alphaCTD, and alphaCTD makes direct protein-DNA interactions with the DNA segment adjacent to the DNA site for CAP. There are no large-scale conformational changes in CAP and alphaCTD, and the interface between CAP and alphaCTD is small. These findings are consistent with the proposal that activation involves a simple "recruitment" mechanism.
About this Structure
1LB2 is a Protein complex structure of sequences from Escherichia coli with as ligand. The following page contains interesting information on the relation of 1LB2 with [Catabolite Activator Protein]. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structural basis of transcription activation: the CAP-alpha CTD-DNA complex., Benoff B, Yang H, Lawson CL, Parkinson G, Liu J, Blatter E, Ebright YW, Berman HM, Ebright RH, Science. 2002 Aug 30;297(5586):1562-6. PMID:12202833
Page seeded by OCA on Thu Feb 21 13:43:15 2008
