1lbg
From Proteopedia
(New page: 200px<br /><applet load="1lbg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lbg, resolution 4.800Å" /> '''LACTOSE OPERON REPR...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lbg.gif|left|200px]]<br /><applet load="1lbg" size=" | + | [[Image:1lbg.gif|left|200px]]<br /><applet load="1lbg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lbg, resolution 4.800Å" /> | caption="1lbg, resolution 4.800Å" /> | ||
'''LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY'''<br /> | '''LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The lac operon of Escherichia coli is the paradigm for gene regulation. | + | The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1LBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1LBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LBG OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chang, G.]] | [[Category: Chang, G.]] | ||
| - | [[Category: Horton, N | + | [[Category: Horton, N C.]] |
| - | [[Category: Kercher, M | + | [[Category: Kercher, M A.]] |
[[Category: Lewis, M.]] | [[Category: Lewis, M.]] | ||
[[Category: Lu, P.]] | [[Category: Lu, P.]] | ||
| - | [[Category: Pace, H | + | [[Category: Pace, H C.]] |
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
[[Category: repressor]] | [[Category: repressor]] | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:23 2008'' |
Revision as of 11:43, 21 February 2008
|
LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY
Overview
The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.
About this Structure
1LBG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer., Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P, Science. 1996 Mar 1;271(5253):1247-54. PMID:8638105
Page seeded by OCA on Thu Feb 21 13:43:23 2008
