1lcn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1lcn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lcn, resolution 1.63&Aring;" /> '''Monoclinic hen egg w...)
Line 1: Line 1:
-
[[Image:1lcn.jpg|left|200px]]<br /><applet load="1lcn" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1lcn.jpg|left|200px]]<br /><applet load="1lcn" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1lcn, resolution 1.63&Aring;" />
caption="1lcn, resolution 1.63&Aring;" />
'''Monoclinic hen egg white lysozyme, thiocyanate complex'''<br />
'''Monoclinic hen egg white lysozyme, thiocyanate complex'''<br />
==Overview==
==Overview==
-
Understanding direct salt effects on protein crystal polymorphism is, addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human, lysozymes. Four new structures of hen egg-white lysozyme are reported:, crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to, 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures, are compared with previously published structures in order to draw a, mapping of the surface of different lysozymes interacting with monovalent, anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An, analysis of the structural sites of these anions in the various lysozyme, structures is presented. This study shows common anion sites whatever the, crystal form and the chemical nature of anions, while others seem specific, to a given geometry and a particular charge environment induced by the, crystal packing.
+
Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.
==About this Structure==
==About this Structure==
-
1LCN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with SCN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LCN OCA].
+
1LCN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=SCN:'>SCN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCN OCA].
==Reference==
==Reference==
Line 17: Line 17:
[[Category: Hamiaux, C.]]
[[Category: Hamiaux, C.]]
[[Category: Prange, T.]]
[[Category: Prange, T.]]
-
[[Category: Vaney, M.C.]]
+
[[Category: Vaney, M C.]]
[[Category: SCN]]
[[Category: SCN]]
[[Category: glycosidase]]
[[Category: glycosidase]]
Line 23: Line 23:
[[Category: lysozyme]]
[[Category: lysozyme]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:28:45 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:47 2008''

Revision as of 11:43, 21 February 2008

Image:1lcn.jpg

1lcn, resolution 1.63Å

Drag the structure with the mouse to rotate

Monoclinic hen egg white lysozyme, thiocyanate complex

Overview

Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.

About this Structure

1LCN is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.

Reference

Structural effects of monovalent anions on polymorphic lysozyme crystals., Vaney MC, Broutin I, Retailleau P, Douangamath A, Lafont S, Hamiaux C, Prange T, Ducruix A, Ries-Kautt M, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):929-40. Epub 2001, Jun 21. PMID:11418760

Page seeded by OCA on Thu Feb 21 13:43:47 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lcn"
Personal tools