1lcy
From Proteopedia
(New page: 200px<br /> <applet load="1lcy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lcy, resolution 2.00Å" /> '''Crystal Structure o...) |
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| - | [[Image:1lcy.gif|left|200px]]<br /> | + | [[Image:1lcy.gif|left|200px]]<br /><applet load="1lcy" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1lcy, resolution 2.00Å" /> | caption="1lcy, resolution 2.00Å" /> | ||
'''Crystal Structure of the Mitochondrial Serine Protease HtrA2'''<br /> | '''Crystal Structure of the Mitochondrial Serine Protease HtrA2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | HtrA2/Omi, a mitochondrial serine protease in mammals, is important in | + | HtrA2/Omi, a mitochondrial serine protease in mammals, is important in programmed cell death. However, the underlining mechanism of HtrA2/Omi-mediated apoptosis remains unclear. Analogous to the bacterial homolog HtrA (DegP), the mature HtrA2 protein contains a central serine protease domain and a C-terminal PDZ domain. The 2.0 A crystal structure of HtrA2/Omi reveals the formation of a pyramid-shaped homotrimer mediated exclusively by the serine protease domains. The peptide-binding pocket of the PDZ domain is buried in the intimate interface between the PDZ and the protease domains. Mutational analysis reveals that the monomeric HtrA2/Omi mutants are unable to induce cell death and are deficient in protease activity. The PDZ domain modulates HtrA2/Omi-mediated cell death activity by regulating its serine protease activity. These structural and biochemical observations provide an important framework for deciphering the mechanisms of HtrA2/Omi-mediated apoptosis. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1LCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alnemri, E | + | [[Category: Alnemri, E S.]] |
[[Category: Chai, J.]] | [[Category: Chai, J.]] | ||
[[Category: Li, P.]] | [[Category: Li, P.]] | ||
[[Category: Li, W.]] | [[Category: Li, W.]] | ||
[[Category: Shi, Y.]] | [[Category: Shi, Y.]] | ||
| - | [[Category: Srinivasula, S | + | [[Category: Srinivasula, S M.]] |
| - | [[Category: Wu, J | + | [[Category: Wu, J W.]] |
[[Category: Zhang, Z.]] | [[Category: Zhang, Z.]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:55 2008'' |
Revision as of 11:44, 21 February 2008
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Crystal Structure of the Mitochondrial Serine Protease HtrA2
Contents |
Overview
HtrA2/Omi, a mitochondrial serine protease in mammals, is important in programmed cell death. However, the underlining mechanism of HtrA2/Omi-mediated apoptosis remains unclear. Analogous to the bacterial homolog HtrA (DegP), the mature HtrA2 protein contains a central serine protease domain and a C-terminal PDZ domain. The 2.0 A crystal structure of HtrA2/Omi reveals the formation of a pyramid-shaped homotrimer mediated exclusively by the serine protease domains. The peptide-binding pocket of the PDZ domain is buried in the intimate interface between the PDZ and the protease domains. Mutational analysis reveals that the monomeric HtrA2/Omi mutants are unable to induce cell death and are deficient in protease activity. The PDZ domain modulates HtrA2/Omi-mediated cell death activity by regulating its serine protease activity. These structural and biochemical observations provide an important framework for deciphering the mechanisms of HtrA2/Omi-mediated apoptosis.
Disease
Known diseases associated with this structure: Parkinson disease 13 OMIM:[606441]
About this Structure
1LCY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural insights into the pro-apoptotic function of mitochondrial serine protease HtrA2/Omi., Li W, Srinivasula SM, Chai J, Li P, Wu JW, Zhang Z, Alnemri ES, Shi Y, Nat Struct Biol. 2002 Jun;9(6):436-41. PMID:11967569
Page seeded by OCA on Thu Feb 21 13:43:55 2008
Categories: Homo sapiens | Single protein | Alnemri, E S. | Chai, J. | Li, P. | Li, W. | Shi, Y. | Srinivasula, S M. | Wu, J W. | Zhang, Z. | Apoptosis | Caspase activation | Iap-binding | Pdz domain | Serine protease
