1ljx
From Proteopedia
(New page: 200px<br /><applet load="1ljx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ljx, resolution 1.64Å" /> '''THE STRUCTURE OF D(T...) |
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- | [[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size=" | + | [[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ljx, resolution 1.64Å" /> | caption="1ljx, resolution 1.64Å" /> | ||
'''THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE'''<br /> | '''THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE'''<br /> | ||
==Overview== | ==Overview== | ||
- | The crystal structure of a hexameric DNA fragment with the sequence | + | The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment. |
==About this Structure== | ==About this Structure== | ||
- | 1LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gautham, N.]] | [[Category: Gautham, N.]] | ||
- | [[Category: Kumar, P | + | [[Category: Kumar, P Satheesh.]] |
- | [[Category: Rajan, S | + | [[Category: Rajan, S S.]] |
[[Category: Thiyagarajan, S.]] | [[Category: Thiyagarajan, S.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: z-dna hexamer with at base pairs at the terminal]] | [[Category: z-dna hexamer with at base pairs at the terminal]] | ||
- | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:39 2008'' |
Revision as of 11:45, 21 February 2008
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THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE
Overview
The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.
About this Structure
1LJX is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature., Thiyagarajan S, Satheesh Kumar P, Rajan SS, Gautham N, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1381-4. Epub 2002, Jul 20. PMID:12136163
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