1lk2
From Proteopedia
(New page: 200px<br /><applet load="1lk2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lk2, resolution 1.35Å" /> '''1.35A crystal struct...) |
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| - | [[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size=" | + | [[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lk2, resolution 1.35Å" /> | caption="1lk2, resolution 1.35Å" /> | ||
'''1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide'''<br /> | '''1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We identify and consider some characteristics of a peptide antagonist for | + | We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG, PO4, MRD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=MRD:'>MRD</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LK2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Eisen, H.]] | [[Category: Eisen, H.]] | ||
| - | [[Category: Luz, J | + | [[Category: Luz, J G.]] |
| - | [[Category: Rudolph, M | + | [[Category: Rudolph, M G.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: MPD]] | [[Category: MPD]] | ||
[[Category: MRD]] | [[Category: MRD]] | ||
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[[Category: high resolution]] | [[Category: high resolution]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:41 2008'' |
Revision as of 11:45, 21 February 2008
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1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide
Overview
We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.
About this Structure
1LK2 is a Protein complex structure of sequences from Mus musculus with , , and as ligands. Full crystallographic information is available from OCA.
Reference
A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects., Rudolph MG, Shen LQ, Lamontagne SA, Luz JG, Delaney JR, Ge Q, Cho BK, Palliser D, McKinley CA, Chen J, Wilson IA, Eisen HN, J Immunol. 2004 Mar 1;172(5):2994-3002. PMID:14978103
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