This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lkb
From Proteopedia
(New page: 200px<br /><applet load="1lkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lkb, resolution 1.70Å" /> '''Porcine Pancreatic E...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lkb.jpg|left|200px]]<br /><applet load="1lkb" size=" | + | [[Image:1lkb.jpg|left|200px]]<br /><applet load="1lkb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lkb, resolution 1.70Å" /> | caption="1lkb, resolution 1.70Å" /> | ||
'''Porcine Pancreatic Elastase/Na-Complex'''<br /> | '''Porcine Pancreatic Elastase/Na-Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Porcine pancreatic elastase has been crystallized at slightly acidic pH | + | Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences. |
==About this Structure== | ==About this Structure== | ||
| - | 1LKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4, CL and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http:// | + | 1LKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LKB OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Nowak, E.]] | [[Category: Nowak, E.]] | ||
[[Category: Panjikar, S.]] | [[Category: Panjikar, S.]] | ||
| - | [[Category: Tucker, P | + | [[Category: Tucker, P A.]] |
| - | [[Category: Weiss, M | + | [[Category: Weiss, M S.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: NA]] | [[Category: NA]] | ||
| Line 27: | Line 27: | ||
[[Category: sulfate binding]] | [[Category: sulfate binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:43 2008'' |
Revision as of 11:45, 21 February 2008
|
Porcine Pancreatic Elastase/Na-Complex
Overview
Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
About this Structure
1LKB is a Single protein structure of sequence from Sus scrofa with , and as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.
Reference
Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296
Page seeded by OCA on Thu Feb 21 13:45:43 2008
