1lmb
From Proteopedia
(New page: 200px<br /><applet load="1lmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lmb, resolution 1.800Å" /> '''REFINED 1.8 ANGSTRO...) |
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| - | [[Image:1lmb.gif|left|200px]]<br /><applet load="1lmb" size=" | + | [[Image:1lmb.gif|left|200px]]<br /><applet load="1lmb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lmb, resolution 1.800Å" /> | caption="1lmb, resolution 1.800Å" /> | ||
'''REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX'''<br /> | '''REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the lambda repressor-operator complex has been | + | The crystal structure of the lambda repressor-operator complex has been refined to an R-factor of 18.9% at 1.8 A resolution. This refinement, using data collected at low temperature, has revealed the structure of the N-terminal arm and shows that the interactions of repressor with the two halves of the pseudo-symmetric operator site are significantly different. The two halves of the complex are most similar near the outer edge of the operator site (in a region where the lambda and 434 repressors make similar contacts), but they become increasingly different toward the center of the operator. There are striking differences near the center of the site where it appears that the arm makes significant contacts to only one half of the DNA site. This suggested a new way of aligning the operator sites in phage lambda. The high resolution structure confirms many of the previously noted features of the complex, but also reveals a number of new protein-DNA contacts. It also gives a better view of the extensive H-bonding networks that couple contacts made by different residues and different regions of the protein, and reveals important new details about the helix-turn-helix (HTH) region, and the positions of many water molecules in the complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1LMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. This structure | + | 1LMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. This structure supersedes the now removed PDB entry 1LRD. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Beamer, L | + | [[Category: Beamer, L J.]] |
| - | [[Category: Pabo, C | + | [[Category: Pabo, C O.]] |
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:18 2008'' |
Revision as of 11:46, 21 February 2008
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REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX
Overview
The crystal structure of the lambda repressor-operator complex has been refined to an R-factor of 18.9% at 1.8 A resolution. This refinement, using data collected at low temperature, has revealed the structure of the N-terminal arm and shows that the interactions of repressor with the two halves of the pseudo-symmetric operator site are significantly different. The two halves of the complex are most similar near the outer edge of the operator site (in a region where the lambda and 434 repressors make similar contacts), but they become increasingly different toward the center of the operator. There are striking differences near the center of the site where it appears that the arm makes significant contacts to only one half of the DNA site. This suggested a new way of aligning the operator sites in phage lambda. The high resolution structure confirms many of the previously noted features of the complex, but also reveals a number of new protein-DNA contacts. It also gives a better view of the extensive H-bonding networks that couple contacts made by different residues and different regions of the protein, and reveals important new details about the helix-turn-helix (HTH) region, and the positions of many water molecules in the complex.
About this Structure
1LMB is a Single protein structure of sequence from Enterobacteria phage lambda. This structure supersedes the now removed PDB entry 1LRD. Full crystallographic information is available from OCA.
Reference
Refined 1.8 A crystal structure of the lambda repressor-operator complex., Beamer LJ, Pabo CO, J Mol Biol. 1992 Sep 5;227(1):177-96. PMID:1387915
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