1lmr
From Proteopedia
(New page: 200px<br /><applet load="1lmr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lmr" /> '''Solution of ADO1, a Toxin from the Assassin ...) |
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| - | [[Image:1lmr.gif|left|200px]]<br /><applet load="1lmr" size=" | + | [[Image:1lmr.gif|left|200px]]<br /><applet load="1lmr" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution of ADO1, a Toxin from the Assassin Bugs Agriosphodrus dohrni that Blocks the Voltage Sensitive Calcium Channel L-type'''<br /> | '''Solution of ADO1, a Toxin from the Assassin Bugs Agriosphodrus dohrni that Blocks the Voltage Sensitive Calcium Channel L-type'''<br /> | ||
==Overview== | ==Overview== | ||
| - | ADO1 is a toxin purified from the saliva of the assassin bug, Agriosphodrus dohrni. Because of its similarity in sequence to Ptu1 from | + | ADO1 is a toxin purified from the saliva of the assassin bug, Agriosphodrus dohrni. Because of its similarity in sequence to Ptu1 from another assassin bug, we did not assess its pharmacologic target. Here, we demonstrate by electrophysiologic means that ADO1 targets the P/Q-type voltage-sensitive calcium channel. We also determine the solution structure of ADO1 using two-dimensional NMR techniques, followed by distance geometry and molecular dynamics. The structure of ADO1 belongs to the inhibitory cystine knot (ICK) structural family (i.e., a compact disulfide-bonded core from which four loops emerge). ADO1 contains a two-stranded, antiparallel beta-sheet structure. We compare the structure of ADO1 with other voltage-sensitive calcium-channel blockers, analyze the topologic juxtaposition of key functional residues, and conclude that the recognition of voltage-sensitive calcium channels by toxins belonging to the ICK structural family requires residues located on two distinct areas of the molecular surface of the toxins. |
==About this Structure== | ==About this Structure== | ||
| - | 1LMR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agriosphodrus_dohrni Agriosphodrus dohrni]. Full crystallographic information is available from [http:// | + | 1LMR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agriosphodrus_dohrni Agriosphodrus dohrni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ick]] | [[Category: ick]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:23 2008'' |
Revision as of 11:46, 21 February 2008
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Solution of ADO1, a Toxin from the Assassin Bugs Agriosphodrus dohrni that Blocks the Voltage Sensitive Calcium Channel L-type
Overview
ADO1 is a toxin purified from the saliva of the assassin bug, Agriosphodrus dohrni. Because of its similarity in sequence to Ptu1 from another assassin bug, we did not assess its pharmacologic target. Here, we demonstrate by electrophysiologic means that ADO1 targets the P/Q-type voltage-sensitive calcium channel. We also determine the solution structure of ADO1 using two-dimensional NMR techniques, followed by distance geometry and molecular dynamics. The structure of ADO1 belongs to the inhibitory cystine knot (ICK) structural family (i.e., a compact disulfide-bonded core from which four loops emerge). ADO1 contains a two-stranded, antiparallel beta-sheet structure. We compare the structure of ADO1 with other voltage-sensitive calcium-channel blockers, analyze the topologic juxtaposition of key functional residues, and conclude that the recognition of voltage-sensitive calcium channels by toxins belonging to the ICK structural family requires residues located on two distinct areas of the molecular surface of the toxins.
About this Structure
1LMR is a Single protein structure of sequence from Agriosphodrus dohrni. Full crystallographic information is available from OCA.
Reference
Solution structure of ADO1, a toxin extracted from the saliva of the assassin bug, Agriosphodrus dohrni., Bernard C, Corzo G, Adachi-Akahane S, Foures G, Kanemaru K, Furukawa Y, Nakajima T, Darbon H, Proteins. 2004 Feb 1;54(2):195-205. PMID:14696181
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