1lnl
From Proteopedia
(New page: 200px<br /><applet load="1lnl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lnl, resolution 3.30Å" /> '''Structure of deoxyge...) |
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| - | [[Image:1lnl.gif|left|200px]]<br /><applet load="1lnl" size=" | + | [[Image:1lnl.gif|left|200px]]<br /><applet load="1lnl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lnl, resolution 3.30Å" /> | caption="1lnl, resolution 3.30Å" /> | ||
'''Structure of deoxygenated hemocyanin from Rapana thomasiana'''<br /> | '''Structure of deoxygenated hemocyanin from Rapana thomasiana'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structure-function relationships in a molluscan hemocyanin have been | + | Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form. |
==About this Structure== | ==About this Structure== | ||
| - | 1LNL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rapana_thomasiana Rapana thomasiana] with NAG and CU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LNL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rapana_thomasiana Rapana thomasiana] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Betzel, C.]] | [[Category: Betzel, C.]] | ||
[[Category: Genov, N.]] | [[Category: Genov, N.]] | ||
| - | [[Category: Guthoehrlein, E | + | [[Category: Guthoehrlein, E W.]] |
[[Category: Idakieva, K.]] | [[Category: Idakieva, K.]] | ||
[[Category: Perbandt, M.]] | [[Category: Perbandt, M.]] | ||
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[[Category: oxygen]] | [[Category: oxygen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:34 2008'' |
Revision as of 11:46, 21 February 2008
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Structure of deoxygenated hemocyanin from Rapana thomasiana
Overview
Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form.
About this Structure
1LNL is a Single protein structure of sequence from Rapana thomasiana with and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:12767214
Page seeded by OCA on Thu Feb 21 13:46:34 2008
Categories: Rapana thomasiana | Single protein | Betzel, C. | Genov, N. | Guthoehrlein, E W. | Idakieva, K. | Perbandt, M. | Rypniewski, W. | Stoeva, S. | Voelter, W. | CU | NAG | Cooperativity | Copper | Deoxygenated form | Hemocyanin | Metal binding site | Oxygen
