1lns
From Proteopedia
(New page: 200px<br /><applet load="1lns" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lns, resolution 2.20Å" /> '''Crystal Structure An...) |
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| - | [[Image:1lns.jpg|left|200px]]<br /><applet load="1lns" size=" | + | [[Image:1lns.jpg|left|200px]]<br /><applet load="1lns" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lns, resolution 2.20Å" /> | caption="1lns, resolution 2.20Å" /> | ||
'''Crystal Structure Analysis of the X-Prolyl Dipeptidyl Aminopeptidase From Lactococcus lactis'''<br /> | '''Crystal Structure Analysis of the X-Prolyl Dipeptidyl Aminopeptidase From Lactococcus lactis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-prolyl dipeptidyl aminopeptidase (X-PDAP) from Lactococcus lactis is | + | The X-prolyl dipeptidyl aminopeptidase (X-PDAP) from Lactococcus lactis is a dimeric enzyme catalyzing the removal of Xaa-Pro dipeptides from the N terminus of peptides. The structure of the enzyme was solved at 2.2 A resolution and provides a model for the peptidase family S15. Each monomer is composed of four domains. The larger one presents an alpha/beta hydrolase fold and comprises the active site serine. The specificity pocket is mainly built by residues from a small helical domain which is, together with the N-terminal domain, essential for dimerization. A C-terminal moiety probably plays a role in the tropism of X-PDAP toward the cellular membrane. These results give new insights for further exploration of the role of the enzymes of the SC clan. |
==About this Structure== | ==About this Structure== | ||
| - | 1LNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Active as [http://en.wikipedia.org/wiki/Xaa-Pro_dipeptidyl-peptidase Xaa-Pro dipeptidyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.14.11 3.4.14.11] Full crystallographic information is available from [http:// | + | 1LNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Active as [http://en.wikipedia.org/wiki/Xaa-Pro_dipeptidyl-peptidase Xaa-Pro dipeptidyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.14.11 3.4.14.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xaa-Pro dipeptidyl-peptidase]] | [[Category: Xaa-Pro dipeptidyl-peptidase]] | ||
| - | [[Category: Chich, J | + | [[Category: Chich, J F.]] |
| - | [[Category: Delage, M | + | [[Category: Delage, M M.]] |
[[Category: Mechin, I.]] | [[Category: Mechin, I.]] | ||
[[Category: Rigolet, P.]] | [[Category: Rigolet, P.]] | ||
[[Category: alpha beta hydrolase fold]] | [[Category: alpha beta hydrolase fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:44 2008'' |
Revision as of 11:46, 21 February 2008
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Crystal Structure Analysis of the X-Prolyl Dipeptidyl Aminopeptidase From Lactococcus lactis
Overview
The X-prolyl dipeptidyl aminopeptidase (X-PDAP) from Lactococcus lactis is a dimeric enzyme catalyzing the removal of Xaa-Pro dipeptides from the N terminus of peptides. The structure of the enzyme was solved at 2.2 A resolution and provides a model for the peptidase family S15. Each monomer is composed of four domains. The larger one presents an alpha/beta hydrolase fold and comprises the active site serine. The specificity pocket is mainly built by residues from a small helical domain which is, together with the N-terminal domain, essential for dimerization. A C-terminal moiety probably plays a role in the tropism of X-PDAP toward the cellular membrane. These results give new insights for further exploration of the role of the enzymes of the SC clan.
About this Structure
1LNS is a Single protein structure of sequence from Lactococcus lactis. Active as Xaa-Pro dipeptidyl-peptidase, with EC number 3.4.14.11 Full crystallographic information is available from OCA.
Reference
The structural basis for catalysis and specificity of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis., Rigolet P, Mechin I, Delage MM, Chich JF, Structure. 2002 Oct;10(10):1383-94. PMID:12377124
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