1lo3
From Proteopedia
(New page: 200px<br /> <applet load="1lo3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lo3, resolution 2.30Å" /> '''Retro-Diels-Alderas...) |
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| - | [[Image:1lo3.gif|left|200px]]<br /> | + | [[Image:1lo3.gif|left|200px]]<br /><applet load="1lo3" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lo3" size=" | + | |
caption="1lo3, resolution 2.30Å" /> | caption="1lo3, resolution 2.30Å" /> | ||
'''Retro-Diels-Alderase Catalytic Antibody: Product Analogue'''<br /> | '''Retro-Diels-Alderase Catalytic Antibody: Product Analogue'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The nitroxyl synthase catalytic antibodies 10F11, 9D9, and 27C5 catalyze | + | The nitroxyl synthase catalytic antibodies 10F11, 9D9, and 27C5 catalyze the release of nitroxyl from a bicyclic pro-drug by accelerating a retro-Diels-Alder reaction. The Fabs (antigen-binding fragments) of these three catalytic antibodies were cloned and sequenced. Fab 9D9 was crystallized in the apo-form and in complex with one transition state analogue of the reaction. Crystal structures of Fab 10F11 in complex with ligands mimicking substrate, transition state, and product have been determined at resolutions ranging from 1.8 to 2.3 A. Antibodies 9D9 and 10F11 show increased shape complementarity (as quantified by the program sc) to the hapten and to a modeled transition state as compared with substrate and product. The shape complementarity is mediated to a large extent by an aromatic residue (tyrosine or tryptophan) at the bottom of the hydrophobic active pocket, which undergoes pi-stacking interactions with the aromatic rings of the ligands. Another factor contributing to the different reactivity of the regioisomers probably arises because of hydrogen-bonding interactions between the nitroxyl bridge and the backbone amide of PheH101 and possibly a conserved water molecule. |
==About this Structure== | ==About this Structure== | ||
| - | 1LO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with AN1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=AN1:'>AN1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LO3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Baumann, U.]] | [[Category: Baumann, U.]] | ||
[[Category: Hugot, M.]] | [[Category: Hugot, M.]] | ||
| - | [[Category: Reymond, J | + | [[Category: Reymond, J L.]] |
[[Category: AN1]] | [[Category: AN1]] | ||
[[Category: catalytic antibody]] | [[Category: catalytic antibody]] | ||
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[[Category: product complex]] | [[Category: product complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:42 2008'' |
Revision as of 11:46, 21 February 2008
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Retro-Diels-Alderase Catalytic Antibody: Product Analogue
Overview
The nitroxyl synthase catalytic antibodies 10F11, 9D9, and 27C5 catalyze the release of nitroxyl from a bicyclic pro-drug by accelerating a retro-Diels-Alder reaction. The Fabs (antigen-binding fragments) of these three catalytic antibodies were cloned and sequenced. Fab 9D9 was crystallized in the apo-form and in complex with one transition state analogue of the reaction. Crystal structures of Fab 10F11 in complex with ligands mimicking substrate, transition state, and product have been determined at resolutions ranging from 1.8 to 2.3 A. Antibodies 9D9 and 10F11 show increased shape complementarity (as quantified by the program sc) to the hapten and to a modeled transition state as compared with substrate and product. The shape complementarity is mediated to a large extent by an aromatic residue (tyrosine or tryptophan) at the bottom of the hydrophobic active pocket, which undergoes pi-stacking interactions with the aromatic rings of the ligands. Another factor contributing to the different reactivity of the regioisomers probably arises because of hydrogen-bonding interactions between the nitroxyl bridge and the backbone amide of PheH101 and possibly a conserved water molecule.
About this Structure
1LO3 is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
A structural basis for the activity of retro-Diels-Alder catalytic antibodies: evidence for a catalytic aromatic residue., Hugot M, Bensel N, Vogel M, Reymond MT, Stadler B, Reymond JL, Baumann U, Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9674-8. Epub 2002 Jul 1. PMID:12093912
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