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1lta
From Proteopedia
(New page: 200px<br /><applet load="1lta" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lta, resolution 2.2Å" /> '''2.2 ANGSTROMS CRYSTAL...) |
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| - | [[Image:1lta.jpg|left|200px]]<br /><applet load="1lta" size=" | + | [[Image:1lta.jpg|left|200px]]<br /><applet load="1lta" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lta, resolution 2.2Å" /> | caption="1lta, resolution 2.2Å" /> | ||
'''2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE'''<br /> | '''2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The galactose-binding site in cholera toxin and the closely related | + | The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1LTA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LTA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTA OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Hol, W | + | [[Category: Hol, W G.J.]] |
| - | [[Category: Kalk, K | + | [[Category: Kalk, K H.]] |
| - | [[Category: Merritt, E | + | [[Category: Merritt, E A.]] |
| - | [[Category: Sixma, T | + | [[Category: Sixma, T K.]] |
| - | [[Category: Zanten, B | + | [[Category: Zanten, B A.M Van.]] |
[[Category: GAL]] | [[Category: GAL]] | ||
[[Category: enterotoxin]] | [[Category: enterotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:13 2008'' |
Revision as of 11:48, 21 February 2008
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2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE
Overview
The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.
About this Structure
1LTA is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT)., Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG, Mol Microbiol. 1994 Aug;13(4):745-53. PMID:7997185
Page seeded by OCA on Thu Feb 21 13:48:13 2008
